Clin Exp Reprod Med.  2018 Sep;45(3):110-115. 10.5653/cerm.2018.45.3.110.

Rapid freezing versus Cryotop vitrification of mouse two-cell embryos

Affiliations
  • 1Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand. tvutyava@gmail.com

Abstract


OBJECTIVE
To compare our in-house method of embryo freezing with Cryotop vitrification in terms of immediate survival, subsequent cleavage and blastocyst formation, and cell numbers in blastocysts.
METHODS
Two-cell mouse embryos were randomly allocated into three groups: a non-frozen control group (group 1, n=300), a group that underwent Cryotop vitrification (group 2, n=300), and a group that underwent our in-house freezing method (group 3, n=300).
RESULTS
There were no significant differences between groups 2 and 3 in the immediate survival rate (96.3% vs. 98.6%, respectively; p=0.085), the further cleavage rate (91.7% vs. 95.0%, respectively; p=0.099), or the blastocyst formation rate (80.7% vs. 78.6%, respectively; p=0.437). The cell numbers in the blastocysts from groups 1, 2, and 3 were comparable (88.99±10.44, 88.29±14.79, and 86.42±15.23, respectively; p=0.228). However, the percentage of good-quality blastocysts in the Cryotop vitrification group was significantly higher than in the group in which our in-house method was performed, but was lower than in the control group (58.0%, 37.0%, and 82.7%, respectively; p < 0.001).
CONCLUSION
At present, our method is inferior to the commercial Cryotop vitrification system. However, with further improvements, it has the potential to be useful in routine practice, as it is easier to perform than the current vitrification system.

Keyword

Cryo-survival rate, Cryotop vitrification; Embryo cryopreservation; Rapid freezing method

MeSH Terms

Animals
Blastocyst
Cell Count
Embryonic Structures*
Freezing*
Methods
Mice*
Survival Rate
Vitrification*
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