Korean J Parasitol.  2018 Jun;56(3):275-280. 10.3347/kjp.2018.56.3.275.

Genetic and Morphologic Identification of Spirometra ranarum in Myanmar

Affiliations
  • 1Department of Parasitology, Parasite Research Center and Parasite Resource Bank, Chungbuk National University School of Medicine, Cheongju 28644, Korea. kseom@chungbuk.ac.kr
  • 2Department of Parasitology and Tropical Medicine, Institute of Health Sciences, Gyeongsang National University College of Medicine, Chinju 52727, Korea.
  • 3Department of Medical Environmental Biology, Chung-Ang University College of Medicine, Seoul 06974, Korea.
  • 4Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 03080, Korea.

Abstract

In the present study, we identified a Spirometra species of Myanmar origin (plerocercoid) by molecular analysis using mitochondrial cox1 and nad1 genes, as well as by morphological observations of an adult tapeworm. Spargana specimens were collected from a paddy-field in Taik Kyi Township Tarkwa Village, Yangon, Myanmar in December 2017. A total of 5 spargana were obtained from 20 frogs Hoplobatrachus rugulosus; syn: Rana rugulosa (Wiegmann, 1834) or R. tigrina (Steindachner, 1867). The plerocercoids were used for experimental infection of a dog. After 4 weeks of infection, an adult tapeworm was recovered from the intestine of the dog. Morphologically, the distinct features of Spirometra sp. (Myanmar origin) relative to S. erinaceieuropaei and S. decipiens include a uterine morphology comprising posterior uterine coils that larger than the terminal uterine ball and coiling of the uteri diagonally (swirling) rather than spirally. The cox1 sequences (1,566 bp) of the Myanmar-origin Spirometra species showed 97.9% similarity to a reference sequence of S. decipiens (GenBank no. KJ599679) and 90.5% similarity to a reference sequence of S. erinaceieuropaei (GenBank no. KJ599680). Phylogenetic tree topologies were identical and presented high confidence level of values for the 3 major branches of the 3 Spirometra species in cox1 and nad1 genes. These results indicated that Myanmar-origin Spirometra species coincided with those of S. ranarum and may be considered as a valid species.

Keyword

Spirometra ranarum; spargana; molecular detection; cox1; nad1; Myanmar

MeSH Terms

Adult
Animals
Cestoda
Dogs
Genes, vif
Humans
Intestines
Myanmar*
Ranidae
Spirometra*
Trees
Uterus
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