J Vet Sci.  2016 Sep;17(3):427-429. 10.4142/jvs.2016.17.3.427.

Mycobacterium bovis infection in a wild sow (Sus scrofa): the first case in Korea

Affiliations
  • 1Animal Disease Control Center, Animal and Plant Quarantine Agency, Daegu 42768, Korea. kubk@korea.kr
  • 2Bacterial Disease, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
  • 3Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, Wonju 26493, Korea. bojeon@yonsei.ac.kr
  • 4Gyeongsangnam-do Livestock Veterinary Promotion Institute, Jinju 52733, Korea.
  • 5Gyeongsangbuk-do Veterinary Service Laboratory, Daegu 41405, Korea.

Abstract

Mycobacterium (M.) bovis causes tuberculosis and has a broad host range, including humans, livestock, and wild animals. M. bovis infection of wild boar has been reported in several European countries. We report here the first case of M. bovis infection in a domesticated wild sow in Korea. Granulomatous and necrotizing lesions with small numbers of acid-fast bacilli were observed in nodules of the lung of wild sow. Furthermore, the M. bovis isolate from the wild sow had spoligotype SB0140 and a novel MIRU-VNTR allelic profile, which is not found in cattle and deer in Korea.

Keyword

Korea; Mycobacterium bovis; wild sow

MeSH Terms

Animals
Female
Mycobacterium bovis/*isolation & purification
Republic of Korea
Swine
Swine Diseases/*diagnosis/microbiology/pathology
Tuberculosis/diagnosis/microbiology/pathology/*veterinary

Figure

  • Fig. 1 Visible lesions and Ziehl-Neelsen staining of the lungs of wild sow. (A) Visible lesions in the nodules of the lung from a wild sow showing well-demarcated yellow caseous nodules (arrowheads) at the cut surface. (B) Ziehl–Neelsen staining of a lung section nodule shows acid-fast bacilli (arrows) in a granuloma. Scale bar = 10 µm.

  • Fig. 2 Agarose gel electrophoresis of the polymerase chain reaction products using primers for the rpoB gene (518 bp), RD8 (Mycobacterium [M.] bovis and M. bovis BCG, 360 bp; M. tuberculosis, 150 bp), and RD1 (254 bp). Lane M, 100-bp DNA size marker; Lane 1, wild boar isolate; Lane 2, M. bovis AN5; Lane 3, M. bovis BCG Pasteur; Lane 4, M. tuberculosis H37Rv.


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