Korean J Parasitol.  2018 Apr;56(2):121-127. 10.3347/kjp.2018.56.2.121.

Detection and Identification of Sarcocystis cruzi (Protozoa: Apicomplexa) by Molecular and Ultrastructural Studies in Naturally Infected Korean Cattle (Bos taurus coreanae) from Daejeon, Korea

Affiliations
  • 1College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea. bkpark@cnu.ac.kr
  • 2Department of Biology, Baylor University, Waco, Texas 76798, USA.
  • 3Laboratory of Veterinary Internal Medicine, BK21 PLUS Program for Creative Veterinary Science Research and College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
  • 4College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea.
  • 5College of Veterinary Medicine, Chonbuk National University, Jeonju 54896, Korea.
  • 6College of Animal Science, Kyungpook National University, Daegu 41566, Korea.
  • 7College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
  • 8Laboratory of Veterinary Clinics, National Institute of Animal Science Rural Development Administration, Wanju 55365, Korea.

Abstract

To survey the prevalence of Sarcocystis infections, 210 heart samples were collected from Korean native cattle (Bos taurus coreanae) at an abattoir in Daejeon Metropolitan City, Republic of Korea. Sarcocysts were detected form 31 specimens (14.8%) and identified as Sarcocystis cruzi via transmission electron microscopy. The wall of S. cruzi has flattened protrusions that did not contain fibrils or microfilaments. The protrusions arose irregularly from the base, contained a fine granular substance, lacked internal microfilaments, and measured approximately 0.21-1.25 μm in length and 0.05-0.07 μm in width. Sequence analysis revealed 99.5% homology to S. cruzi. This is the first report on the prevalence of S. cruzi in native cattle from the Republic of Korea.

Keyword

Sarcocystis cruzi; Bos taurus coreanae; ultrastructure; 18S rRNA

MeSH Terms

Abattoirs
Actin Cytoskeleton
Animals
Cattle*
Heart
Korea*
Microscopy, Electron, Transmission
Prevalence
Republic of Korea
Sarcocystis*
Sequence Analysis
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