J Vet Sci.  2017 Jun;18(2):159-167. 10.4142/jvs.2017.18.2.159.

Immunogenicity of recombinant Lactobacillus plantarum NC8 expressing goose parvovirus VP2 gene in BALB/c mice

• Liu YY
• Yang WT
• Shi SH
• Li YJ
• Zhao L
• Shi CW
• Zhou FY
• Jiang YL
• Hu JT
• Gu W
• Yang GL
• Wang CF

• KMID: 2412568
• DOI: http://doi.org/10.4142/jvs.2017.18.2.159

Abstract

Goose parvovirus (GPV) continues to be a threat to goose farms and has significant economic effects on the production of geese. Current commercially available vaccines only rarely prevent GPV infection. In our study, Lactobacillus (L.) plantarum NC8 was selected as a vector to express the VP2 gene of GPV, and recombinant L. plantarum pSIP409-VP2/NC8 was successfully constructed. The molecular weight of the expressed recombinant protein was approximately 70 kDa. Mice were immunized with a 2 × 109 colony-forming unit/200 µL dose of the recombinant L. plantarum strain, and the ratios and numbers of CD11c⁺, CD3⁺CD4⁺, CD3⁺CD8⁺, and interferon gamma- and tumor necrosis factor alpha-expressing spleen lymphocytes in the pSIP409-VP2/NC8 group were higher than those in the control groups. In addition, we assessed the capacity of L. plantarum SIP409-VP2/NC8 to induce secretory IgA production. We conclude that administered pSIP409-VP2/NC8 leads to relatively extensive cellular responses. This study provides information on GPV infection and offers a clear framework of options available for GPV control strategies.

Keyword

Lactobacillus plantarum; VP2 gene; goose parvovirus; immunization

MeSH Terms

Animals
Female
Genes, Viral/genetics
Immunity, Cellular/immunology
Immunoglobulin A, Secretory/immunology
Lactobacillus plantarum/genetics/*immunology/virology
Mice
Mice, Inbred BALB C
Organisms, Genetically Modified
Parvoviridae Infections/prevention & control/veterinary
Parvovirinae/*genetics
Recombinant Proteins/immunology
Vaccines, Synthetic/immunology/pharmacology
Viral Proteins/genetics/*immunology
Viral Vaccines/immunology/pharmacology
Immunoglobulin A, Secretory
Recombinant Proteins
Vaccines, Synthetic
Viral Proteins
Viral Vaccines