J Vet Sci.  2017 Jun;18(2):141-148. 10.4142/jvs.2017.18.2.141.

Effect of donor age on the proliferation and multipotency of canine adipose-derived mesenchymal stem cells

• Lee J
• Lee KS
• Kim CL
• Byeon JS
• Gu NY
• Cho IS
• Cha SH

• KMID: 2412566
• DOI: http://doi.org/10.4142/jvs.2017.18.2.141

Abstract

Research into adipose tissue-derived mesenchymal stem cells (AD-MSCs) has demonstrated the feasibility of their use in clinical applications due to their ease of isolation and abundance in adipose tissue. We isolated AD-MSCs from young and old dogs, and the cells were subjected to sequential sub-passaging from passage 1 (P1) to P7. Canine AD-MSCs (cAD-MSCs) were examined for proliferation kinetics, expression of molecules associated with self-renewal, expression of cell surface markers, and differentiation potentials at P3. Cumulative population doubling level was significantly higher in cAD-MSCs of young donors than in those of old donors. In addition, expressions of CD73, CD80, Oct3/4, Nanog, cell survival genes and differentiation potentials were significantly higher in young donors than in old donors. The present study suggests that donor age should be considered when developing cell-based therapies for clinical application of cAD-MSCs.

Keyword

adipose mesenchymal stem cells; age; canine; differentiation; multipotency

MeSH Terms

Adipose Tissue/*cytology/physiology
Age Factors
Animals
Cell Differentiation/physiology
Cell Proliferation/*physiology
Dogs
Gene Expression Profiling/veterinary
Mesenchymal Stromal Cells/*physiology
Real-Time Polymerase Chain Reaction/veterinary
Reverse Transcriptase Polymerase Chain Reaction/veterinary