Gut Liver.  2016 May;10(3):406-411. 10.5009/gnl15251.

The Effect of DA-6034 on Intestinal Permeability in an Indomethacin-Induced Small Intestinal Injury Model

Affiliations
  • 1Department of Internal Medicine, Hanyang University School of Medicine, Seoul, Korea. leeoy@hanyang.ac.kr

Abstract

BACKGROUND/AIMS
DA-6034 has anti-inflammatory activities and exhibits cytoprotective effects in acute gastric injury models. However, explanations for the protective effects of DA-6034 on intestinal permeability are limited. This study sought to investigate the effect of DA-6034 on intestinal permeability in an indomethacin-induced small intestinal injury model and its protective effect against small intestinal injury.
METHODS
Rats in the treatment group received DA-6034 from days 0 to 2 and indomethacin from days 1 to 2. Rats in the control group received indomethacin from days 1 to 2. On the fourth day, the small intestines were examined to compare the severity of inflammation. Intestinal permeability was evaluated by using fluorescein isothiocyanate-labeled dextran. Western blotting was performed to confirm the association between DA-6034 and the extracellular signal-regulated kinase (ERK) pathway.
RESULTS
The inflammation scores in the treatment group were lower than those in the control group, but the difference was statistically insignificant. Hemorrhagic lesions in the treatment group were broader than those in the control group, but the difference was statistically insignificant. Intestinal permeability was lower in the treatment group than in the control group. DA-6034 enhanced extracellular signal-regulated kinase expression, and intestinal permeability was negatively correlated with ERK expression.
CONCLUSIONS
DA-6034 may decrease intestinal permeability in an indomethacin-induced intestinal injury model via the ERK pathway.

Keyword

DA-6034; Intestinal permeability; Small intestinal injury model; Leaky gut syndrome

MeSH Terms

Administration, Oral
Animals
Anti-Inflammatory Agents, Non-Steroidal/administration & dosage/*pharmacology/toxicity
Disease Models, Animal
Dose-Response Relationship, Drug
Enteritis/chemically induced/physiopathology
Flavonoids/administration & dosage/*pharmacology
Indomethacin/toxicity
Intestine, Small/*drug effects
MAP Kinase Signaling System/drug effects
Permeability/drug effects
Phosphorylation/drug effects
Random Allocation
Rats, Sprague-Dawley
Anti-Inflammatory Agents, Non-Steroidal
Flavonoids
Indomethacin
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