Electrolyte Blood Press.  2010 Dec;8(2):59-65.

Membrane Trafficking of Collecting Duct Water Channel Protein AQP2 Regulated by Akt/AS160

Affiliations
  • 1Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu, Korea. thkwon@knu.ac.kr

Abstract

Akt (protein kinase B (PKB)) is a serine/threonine kinase that acts in the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway. The PI3K/Akt signaling pathway, triggered by growth factors and hormones including vasopressin, is an important pathway that is widely involved in cellular mechanisms regulating transcription, translation, cell growth and death, cell proliferation, migration, and cell cycles. In particular, Akt and Akt substrate protein of 160 kDa (AS160) are likely to participate in the trafficking of aquaporin-2 (AQP2) in the kidney collecting duct. In this study, we demonstrated that 1) small interfering RNA (siRNA)-mediated gene silencing of Akt1 significantly decreased Akt1 and phospho-AS160 protein expression; and 2) confocal laser scanning microscopy of AQP2 in mouse cortical collecting duct cells (M-1 cells) revealed AS160 knockdown by siRNA increased AQP2 expression in the plasma membrane compared with controls, despite the absence of dDAVP stimulation. Thus, the results suggest that PI3K/Akt pathways could play a role in AQP2 trafficking via the AS160 protein.

Keyword

aquaporin 2; protein kinase B; protein transport; rab GTP-binding proteins; TBC1D4 protein

MeSH Terms

Animals
Aquaporin 2
Cell Cycle
Cell Death
Cell Membrane
Deamino Arginine Vasopressin
Gene Silencing
Intercellular Signaling Peptides and Proteins
Kidney Tubules, Collecting
Membranes
Mice
Microscopy, Confocal
Phosphatidylinositol 3-Kinase
Phosphotransferases
Protein Transport
Proto-Oncogene Proteins c-akt
rab GTP-Binding Proteins
RNA, Small Interfering
Vasopressins
Water
Aquaporin 2
Deamino Arginine Vasopressin
Intercellular Signaling Peptides and Proteins
Phosphatidylinositol 3-Kinase
Phosphotransferases
Proto-Oncogene Proteins c-akt
RNA, Small Interfering
Vasopressins
Water
rab GTP-Binding Proteins
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