Ann Surg Treat Res.  2018 Jan;94(1):19-25. 10.4174/astr.2018.94.1.19.

Epigenetic inactivation of RUNX3 in colorectal cancer

Affiliations
  • 1Department of Surgery, Soonchunhyang University College of Medicine, Cheonan, Korea. ssurge@schmc.ac.kr
  • 2Department of Hematology and Oncology, Soonchunhyang University College of Medicine, Cheonan, Korea.
  • 3Department of Obstetrics and Gynecology, Soonchunhyang University College of Medicine, Cheonan, Korea.
  • 4Medical Science Research Institute, Soonchunhyang University, Cheonan, Korea.
  • 5Department of Anatomy, Kyungpook National University School of Medicine, Daegu, Korea.
  • 6Soonchunhyang University College of Medicine, Cheonan, Korea.

Abstract

PURPOSE
Emerging evidence indicates that runt-related transcription factor 3 (RUNX3) is an important tumor suppressor gene in several cancer types, including colorectal cancer (CRC). However, the clinical significance of RUNX3 inactivation in CRC remains unclear. The aim of this study was to examine the correlation between clinicopathologic factors and RUNX3 hypermethylation/expression in CRC.
METHODS
Sixty-two CRC patients who were treated at the Soonchunhyang University College of Medicine were recruited in this study. The hypermethylation of CpG islands in the RUNX3 promoter and the expression of RUNX3 mRNA were identified by methylation-specific polymerase chain reaction (PCR) and reverse transcriptase-PCR, respectively. The expression of RUNX3 was determined by immunohistochemical staining.
RESULTS
Of the 62 CRC tissue samples, 20 (32.3%) presented hypermethylated RUNX3 promoters. Aberrant RUNX3 hypermethylation was found to be associated with vascular (P = 0.006) and lymphatic (P = 0.002) invasion. Hypermethylation of RUNX3 was associated with poor survival outcomes (P = 0.038). However, expression of RUNX3 was not a prognostic factor (P = 0.363).
CONCLUSION
Hypermethylation of RUNX3 may be a predictor of a poor prognosis in CRC.

Keyword

Colorectal neoplasms; Prognosis; RUNX3 protein; Methylation; Immunohistochemistry

MeSH Terms

Colorectal Neoplasms*
Core Binding Factor Alpha 3 Subunit
CpG Islands
Epigenomics*
Genes, Tumor Suppressor
Humans
Immunohistochemistry
Methylation
Polymerase Chain Reaction
Prognosis
RNA, Messenger
Transcription Factor 3
Core Binding Factor Alpha 3 Subunit
RNA, Messenger
Transcription Factor 3
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