Blood Res.  2017 Dec;52(4):316-319. 10.5045/br.2017.52.4.316.

Transplantation of human umbilical cord blood CD34⁺ cells into the liver of newborn NOD/SCID/IL-2Rγ null (NSG) mice after busulfan conditioning

• Ko Y
• Jeong YH
• Lee JA

• KMID: 2405070
• DOI: http://doi.org/10.5045/br.2017.52.4.316

Abstract

No abstract available.

MeSH Terms

Animals
Busulfan*
Fetal Blood*
Humans*
Infant, Newborn*
Liver*
Mice*
Umbilical Cord*
Busulfan

Figure

• 

  Fig. 1 Survival and weight changes of newborn NSG mice after hCD34+ cell injection. Humanized NSG mice were monitored daily from the 3rd week of transplantation. Most of the newborn NSG mice did well, but two showed features suggesting graft versus host disease (weight loss, hunched posture, and diminished activity) and died on the 26th day of transplantation.

• 

  Fig. 2 Reconstitution of human cells in newborn NSG mice after intrahepatic transplantation of CD34+ cells. Mononuclear cells derived from bone marrow, spleen, peripheral blood and liver tissues of humanized NSG mice were isolated in the 12th week of transplantation and were stained with anti-hCD45, anti-hCD3, and anti-hCD19 antibodies. Data are means±SEM and representative of four mice per group, excluding lowest values (N=5).Abbreviations: BM, bone marrow; LV, liver; ND, not detected; PB, peripheral blood; SPL, spleen.

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