Korean J Parasitol.  2017 Dec;55(6):679-684. 10.3347/kjp.2017.55.6.679.

Practical Algorisms for PCR-RFLP-Based Genotyping of Echinococcus granulosus Sensu Lato

Affiliations
  • 1Department of Parasitology and Tropical Medicine, School of Medicine, Pusan National University, Yangsan 50612, Korea. hsyu@pusan.ac.kr
  • 2Immunoregulatory Therapeutics Group in Brain Busan 21 Project, Yonsei University College of Medicine, Seoul 03722, Korea.
  • 3Department of Environmental Medical Biology and Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 03722, Korea.
  • 4Department of Environmental Medical Biology, Yonsei University Wonju College of Medicine, Wonju, 26426, Korea.
  • 5Department of Environmental Medical Biology and Institute for Clinical and Translational Research, Catholic Kwandong University College of Medicine, Gangneung 25601, Korea.
  • 6Isaev Research Institute of Medical Parasitology, Ministry of Health, Republic of Uzbekistan.

Abstract

Echinococcus granulosus sensu lato (s.l.) is a causative agent of cystic echinococcosis or cystic hydatid disease in humans and domestic and wild animals. The disease is a serious health problem in countries associated with poverty and poor hygiene practices, particularly in livestock raising. We introduced a practical algorism for genotyping the parasite, which may be useful to many developing countries. To evaluate the efficiency of the algorism, we genotyped 3 unknown strains isolated from human patients. We found that unknowns 1 and 3 were included in G1, G2, and G3 genotypes group and unknown 2 was included in G4 genotype (Echinococcus equinus) according to the algorisms. We confirmed these results by sequencing the 3 unknown isolates cox1 and nad1 PCR products. In conclusion, these new algorisms are very fast genotype identification tools that are suitable for evaluating E. granulosus s.l. isolated from livestock or livestock holders, particularly in developing countries.

Keyword

Echinococcus granulosus; practical algorism; genotyping; cox1; nad1

MeSH Terms

Animals
Animals, Wild
Developing Countries
Echinococcosis
Echinococcus granulosus*
Echinococcus*
Genotype
Humans
Hygiene
Livestock
Parasites
Polymerase Chain Reaction
Poverty
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