Cancer Res Treat.  2015 Oct;47(4):921-930. 10.4143/crt.2014.153.

Tumor Growth Suppression and Enhanced Radioresponse by an Exogenous Epidermal Growth Factor in Mouse Xenograft Models with A431 Cells

Affiliations
  • 1Department of Radiation Oncology, Seoul National University College of Medicine, Seoul, Korea. wuhg@snu.ac.kr
  • 2Cancer Research Institution, Seoul National University College of Medicine, Seoul, Korea.
  • 3Department of Pathology, Seoul National University College of Medicine, Seoul, Korea.
  • 4Institute of Radiation Medicine, Medical Research Center, Seoul National University, Seoul, Korea.

Abstract

PURPOSE
The purpose of this study was to evaluate whether an exogenous epidermal growth factor (EGF) could induce anti-tumor and radiosensitizing effects in vivo.
MATERIALS AND METHODS
BALB/c-nu mice that were inoculated with A431 (human squamous cell carcinoma) cells in the right hind legs were divided into five groups: I (no treatment), II (EGF for 6 days), III (EGF for 20 days), IV (radiotherapy [RT]), and V (RT plus concomitant EGF). EGF was administered intraperitoneally (5 mg/kg) once a day and the RT dose was 30 Gy in six fractions. Hematoxylin and eosin (H&E) stained sections of tumor, liver, lung, and kidney tissues were investigated. Additionally, tumors were subjected to immunohistochemistry staining with caspase-3.
RESULTS
EGF for 6 days decreased tumor volume, but it approached the level of the control group at the end of follow-up (p=0.550). The duration of tumor shrinkage was prolonged in group V while the slope of tumor re-growth phase was steeper in group IV (p=0.034). EGF for 20 days decreased tumor volume until the end of the observation period (p < 0.001). Immunohistochemistry revealed that mice in group V showed stronger intensity than those in group IV. There were no abnormal histological findings upon H&E staining of the normal organs.
CONCLUSION
EGF-induced anti-tumor effect was ascertained in the xenograft mouse models with A431 cells. Concomitant use of EGF has the potential role as a radiosensitizer in the design of fractionated irradiation.

Keyword

Epidermal growth factor; Radiation-sensitizing agents; Antineoplastic agents; Xenograft model antitumor assays; Apoptosis

MeSH Terms

Animals
Antineoplastic Agents
Apoptosis
Caspase 3
Eosine Yellowish-(YS)
Epidermal Growth Factor*
Follow-Up Studies
Hematoxylin
Heterografts*
Immunohistochemistry
Kidney
Leg
Liver
Lung
Mice*
Radiation-Sensitizing Agents
Tumor Burden
Xenograft Model Antitumor Assays
Antineoplastic Agents
Caspase 3
Eosine Yellowish-(YS)
Epidermal Growth Factor
Hematoxylin
Radiation-Sensitizing Agents
Full Text Links
  • CRT
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error