Clin Exp Vaccine Res.  2018 Jan;7(1):82-86. 10.7774/cevr.2018.7.1.82.

Evaluation of two different enzyme-linked immunosorbent assay for severe fever with thrombocytopenia syndrome virus diagnosis

• Yu MA
• Jeong HW
• Park SJ
• Kim YI
• Kwon HI
• Kim EH
• Si YJ
• Yu KM
• Robles NJ
• Han HJ
• Choi YK

• KMID: 2402542
• DOI: http://doi.org/10.7774/cevr.2018.7.1.82

Abstract

To develop the large scale serological assay for severe fever with thrombocytopenia syndrome virus (SFTSV) infection, we evaluated two different enzyme-linked immunosorbent assay (ELISA) methods using nucleocapsid protein (NP) and Gn proteins of CB1 (genotype B) SFTSV strains. The NP-based ELISA tests showed more sensitive with broad cross-reactivity between two different genotype A and B strains compared with those of Gn-based ELISA tests. However, Gn-based ELISA showed more genotype specificity and specificity. These result suggested that NP-based ELISA test could be applicable for general sero-prevalence studies of SFTSV infections, while Gn-based ELISA could be applicable for a certain specific genotype sero-prevalence study.

Keyword

Severe fever with thrombocytopenia syndrome virus; Enzyme-linked immunosorbent assay; NP protein; Gn protein

MeSH Terms

Diagnosis*
Enzyme-Linked Immunosorbent Assay*
Fever*
Genotype
Nucleocapsid Proteins
Sensitivity and Specificity
Thrombocytopenia*
Nucleocapsid Proteins

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