Nat Prod Sci.  2017 Dec;23(4):227-234. 10.20307/nps.2017.23.4.227.

Induction of Apoptosis with Moringa oleifera Fruits in HCT116 Human Colon Cancer Cells Via Intrinsic Pathway

Affiliations
  • 1College of Natural Sciences, Duksung Women's University, Seoul 01369, Korea. hasook@duksung.ac.kr

Abstract

Moringa oleifera Lam (M. oleifera, Moringaceae) is a tree of the Moringaceae family that can reach a height of between 5 and 10 m. The current paper presents cytotoxic effect of M. oleifera fruits and its flavonoids 1 and 2. The viability of HCT116 human colon cancer cells were 38.5% reduced by 150 µg/mL of ethanolic extracts in a concentration-dependent manner; in addition, we observed the apoptotic features of cell shrinkage and decreased cell size. Bcl-2 family proteins were regulated as determined by Western blotting analysis, suggesting that M. oleifera fruits and their flavonoids 1 and 2 induced apoptosis through an intrinsic pathway. Based on our findings, 70% ethanolic extracts of M. oleifera fruits and flavonoids 1 and 2 might be useful as cytotoxic agents in colorectal cancer therapy.

Keyword

Moringa oleifera fruits; Cytotoxicity; Intrinsic pathway

MeSH Terms

Apoptosis*
Blotting, Western
Cell Size
Colon*
Colonic Neoplasms*
Colorectal Neoplasms
Cytotoxins
Ethanol
Flavonoids
Fruit*
Humans*
Moringa oleifera*
Moringa*
Trees
Cytotoxins
Ethanol
Flavonoids

Figure

  • Fig. 1. Chemical structure of compounds 1 and 2.

  • Fig. 2. Cytotoxic effect of MOF in HCT116 cells. Cell viability at the indicated concentrations was assessed for 72 hr by MTT assay. ∗p < 0.05, significantly different from control cells.

  • Fig. 3. Induction of apoptosis by MOF in HCT116 cells. The formation of apoptotic bodies (arrows) in Hoechst-33258-stained cells observed by fluorescent microscopy.

  • Fig. 4. Induction of apoptosis by MOF in HCT116 cells. Flow cytometric analysis of HCT116 cells incubated with MOF for 72 hr. The right bottom quadrant represents Annexin V-stained cells (early-phase apoptotic cells). The top right quadrant represents PI- and Annexin V-stained cells (late-phase apoptotic cells). ∗p < 0.05, significantly different from control cells.

  • Fig. 5. Regulation of Bcl-2 family in MOF-treated HCT116 cells. Equal amounts of cell lysates were electrophoresed, and Bax and Bcl-2 expression form were detected by Western blotting analysis with corresponding antibodies. ∗p < 0.05, significantly different from control cells.

  • Fig. 6. Cytotoxic effect of compounds 1 and 2 in HCT116 cells. Cell viability at the indicated concentrations was assessed for 72 hr by MTT assay. ∗p < 0.05, significantly different from control cells.

  • Fig. 7. Induction of apoptosis by compounds 1 and 2 in HCT116 cells. The formation of apoptotic bodies (arrows) in Hoechst-33258-stained cells observed by fluorescent microscopy.

  • Fig. 8. Regulation of Bcl-2 family on compounds 1 and 2-treated HCT116 cells. Equal amounts of cell lysates were electrophoresed, and Bax and Bcl-2 expression form were detected by Western blotting analysis with corresponding antibodies. ∗p < 0.05, significantly different from control cells.


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