Mycobiology.  2017 Dec;45(4):434-437. 10.5941/MYCO.2017.45.4.434.

An Improved Total RNA Extraction Method for White Jelly Mushroom Tremella fuciformis Rich in Polysaccharides

Affiliations
  • 1College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. aiminma@mail.hzau.edu.cn
  • 2College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
  • 3Key Laboratory of Agro-Microbial Resources and Utilization, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China.

Abstract

An improved method for extracting high quality and quantity RNA from a jelly mushroom and a dimorphic fungus"”Tremella fuciformis which is especially rich in polysaccharides, is described. RNA was extracted from T. fuciformis mycelium M1332 and its parental monokaryotic yeast-like cells Y13 and Y32. The A260/280 and A260/230 ratios were both approximately 2, and the RNA integrity number was larger than 8.9. The yields of RNA were between 108 and 213 µg/g fresh wt. Downstream molecular applications including reverse transcriptional PCR and quantitative real-time PCR were also performed. This protocol is reliable and may be widely applicable for total RNA extraction from other jelly mushrooms or filamentous fungi rich in polysaccharides.

Keyword

Dimorphism; Jelly mushroom; RNA extraction; Tremella fuciformis

MeSH Terms

Agaricales*
Fungi
Humans
Methods*
Mycelium
Parents
Polymerase Chain Reaction
Polysaccharides*
Real-Time Polymerase Chain Reaction
RNA*
Polysaccharides
RNA
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