Mycobiology.  2017 Dec;45(4):312-317. 10.5941/MYCO.2017.45.4.312.

Differences among Endophytic Fungal Communities Isolated from the Roots of Cephalanthera longibracteata Collected from Different Sites in Korea

Affiliations
  • 1Department of Biology Education, Korea National University of Education, Cheongju 28173, Korea. eomah@knue.ac.kr
  • 2Chungbuk Science High School, Cheongju 28189, Korea.

Abstract

Orchidaceous plants have symbiotic relationships with endophytic fungi, including mycorrhizal fungi, which play important roles in the seed germination and growth of the host plants. In this study, endophytic fungal communities isolated from the roots of Cephalanthera longibracteata collected from three different sites in Korea were analyzed, and it was determined whether fungal communities were preferentially correlated with the sites. The fungal isolates were identified by sequence analysis of the internal transcribed spacer regions of rDNA. In total, 30 species of endophytic fungi, including two species of mycorrhizal fungi belonging to the genus Tulasnella, were identified. Leptodontidium orchidicola showed the highest frequency and was isolated from all root samples. Species diversity and richness were not significantly different among sites. However, the community structure of the endophytic fungi significantly differed among sites, suggesting that the site characteristics affected the community composition of the endophytic fungi colonizing the roots of C. longibracteata. Our findings will aid in developing methods involving the use of symbiotic fungi for orchid conservation and restoration in native habitats.

Keyword

Cephalanthera longibracteata; Fungal endophytes; Leptodontidium orchidicola; Mycorrhizas; Orchids

MeSH Terms

Colon
DNA, Ribosomal
Ecosystem
Fungi
Germination
Korea*
Mycorrhizae
Sequence Analysis
DNA, Ribosomal
Full Text Links
  • MB
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr