J Korean Orthop Assoc.  2004 Aug;39(4):373-379.

Effect of beta-Ig H3 on Chondrogenesis by Human Bone Marrow Mesenchymal Stem Cells

Affiliations
  • 1Department of Orthopedic Surgery, School of Medicine, Catholic University of Daegu, Daegu, Korea. kwkwun@cu.ac.kr
  • 2Department of Laboratory Medicine, School of Medicine, Catholic University of Daegu, Daegu, Korea.
  • 3Department of Biochemistry, School of Medicine, Kyungbook National University, Daegu, Korea.

Abstract

PURPOSE
This study was undertaken to compare the activity of beta-Ig H3 (BigH3) and transforming growth factor (TGF)-beta on chondrogenesis by mesenchymal stem cells (MSCs). MATERIALS AND METHODS: MSCs in human bone marrow aspirated from 20 healthy donors were isolated by density gradient Ficoll-hypaque separation and expanded in culture. MSC-alginate beads were prepared and incubated for 28 days in the presence of 0.5 or 10 ng/mL of TGF-beta 1, TGF-beta 1+TGF-beta 3 or BigH3. Cellular viability, total collagen and glycosaminoglycan (GAG) contents were measured and compared. SPSS version 9.0 was used for the statistical analysis. RESULTS: TGF-beta 3 significantly enhanced cell viability in beads by day 21 (p=0.029). No significant differences were found in terms of cell viability (p=0.197) or in total GAG content (p=0.253) between 10 ng/mL of TGF-beta 1+3 and 10 ng/mL of BigH3. Total collagen content was higher in the BigH3 added group on day 21 (p=0.041). CONCLUSION: The replacement of BigH3 instead of TGF-beta produced appropriate external signals indicating the chondrogenic differentiation of human bone marrow MSCs.

Keyword

Beta-Ig H3; Chondrogenesis; Mesenchymal stem cells; Human bone marrow
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