J Korean Orthop Assoc.  2006 Aug;41(4):643-649.

Matrix Metalloproteinase(MMP)-2 Expression Related to Stretch Injury of the Anterior Cruciate Ligament in the Knee

Affiliations
  • 1Department of Orthopedics, St. Mary's Hospital, The Catholic University, Seoul, Korea. hslee@catholic.ac.kr

Abstract

PURPOSE: Tissue remodeling is essential for the natural healing of damaged tissue. The matrix metalloproteinases (MMPs) that degrade the extracellular matrix to play a central role in this process. This study was designed to examine the basic relationship between MMP-2 and anterior cruciate ligament (ACL) injury.
MATERIALS AND METHODS
After the femur-ACL-tibia complex was harvested from rats, the continuous release of MMP-2 in the stretch group was evaluated. The complexes were divided into three groups. To stretch the complexes, in group I, 5 and 12 N weights were used for 10, 30, and 60 minutes. In group II, 5 and 15 N weights were used for 5, 10, 30, 60, and 120 minutes. And in group III, 5, 8, 10, 12, and 15 N weights were used for 30 and 60 minutes. MMP-2 activity was evaluated using 0.05% gelatin zymography.
RESULTS
MMP-2 was expressed and the signal intensity was increased according to the stretch duration and magnitude. In the tissue analysis, active MMP-2 was markedly present and the expression was increased in response to the stretch in the supernatant samples.
CONCLUSION
MMP-2 was released immediately after ligament injury and its activity was increased in proportion to the stretch duration and magnitude. It is believed that MMP-2 expression plays an important role in the remodeling process after an ACL injury.

Keyword

Knee; Anterior cruciate ligament; Stretch injury; Matrix metalloproteinase

MeSH Terms

Animals
Anterior Cruciate Ligament*
Extracellular Matrix
Gelatin
Knee*
Ligaments
Matrix Metalloproteinases
Rats
Weights and Measures
Gelatin
Matrix Metalloproteinases
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