Ann Clin Microbiol.  2017 Dec;20(4):97-102. 10.5145/ACM.2017.20.4.97.

Evaluation of EuDxâ„¢-PN MLC Detection Kit for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila in Respiratory Specimens

Affiliations
  • 1Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul, Korea. cpworld@cau.ac.kr
  • 2Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
  • 3Seoul Clinical Laboratories (SCL), Yongin, Korea.
  • 4Samkwang Medical Laboratories, Seoul, Korea.

Abstract

BACKGROUND
Infection by the intracellular bacteria Mycoplasma pneumoniae, Chamydophila pneumoniae, and Legionella pneumophila are common causes of community-acquired pneumonia (CAP). This study describes the evaluation of a new multiplex real-time PCR test, EuDxâ„¢-PN MLC Detection Kit (EUDIPIA), which allows the simultaneous detection of M. pneumoniae, C. pneumoniae, and L. pneumophila in respiratory samples.
METHODS
A total of 353 samples were tested using three PCR kits: multiplex PCR (Seeplex PneumoBacter ACE Detection Kit) and two multiplex real-time PCR (EuDxâ„¢-PN MLC Detection Kit and Anyplexâ„¢ II RB5 Detection Kit). The results were considered true positives (expanded standard) for M. pneumoniae, C. pneumoniae, and L. pneumophila if they were positive according to any of the three tests.
RESULTS
The sensitivity and specificity of EuDxâ„¢-PN MLC Detection Kit were 93.3-100% and 100%, respectively. The agreement rate and Cohen's kappa coefficient (value) between EuDxâ„¢-PN MLC Detection Kit and Anyplexâ„¢ II RB5 Detection Kit for M. pneumoniae, C. pneumoniae, and L. pneumophila were 70-100% and 0.82-1, respectively.
CONCLUSION
These results demonstrate that the EuDxâ„¢-PN MLC Detection Kit is a sensitive, specific, and useful screening tool for the detection of atypical pathogens in respiratory samples and can be helpful in selecting appropriate antimicrobial therapy for patients with respiratory infection.

Keyword

Chamydophila pneumoniae; Legionella pneumophila; Real-time polymerase chain reaction; Mycoplasma pneumoniae; Respiratory tract infection
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