Int J Oral Biol.  2017 Sep;42(3):137-142. 10.11620/IJOB.2017.42.3.137.

TNF-α Inhibitor Reduces Odontoclast Formation in Diabetes Rats with Ligature-Induced Periodontitis

Affiliations
  • 1Department of Dental Hygiene, Jeonju kijeon College, Jeonju, Republic of Korea.
  • 2Department of Oral Biology, Yonsei University College of Dentistry, Seoul, Republic of Korea. yu618@yuhs.ac, ejbak@yuhs.ac
  • 3Department of Applied Life Science, The Graduate School, Yonsei University, Seoul, South Korea.
  • 4BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.
  • 5Department of Clinical Laboratory Science, Semyung University, Jecheon, Republic of Korea.

Abstract

To determine the effect of the tumor necrosis factor-α (TNF-α) in odontoclast formation, we administrated a TNF-α inhibitor in rats with diabetes rats with periodontitis. The rats included in the study were divided into three groups: control rats without diabetes or periodontitis (the C group), rats with periodontitis and diabetes (the PD group), and rats with periodontitis and diabetes treated by infliximab, the TNF inhibitor (the PD+infliximab group). The PD and PD+ infliximab groups received intravenous administrations of streptozotocin (STZ, 50 mg/kg) to induce diabetes. After 7 days of STZ injections, the mandibular first molars were ligatured to induce periodontitis. The PD+infliximab group was intrapenitoneally administrated by infliximab (5 mg/kg). On days 3 and 20 after the ligature administration, odontoclast formation along root surfaces was evaluated by tartrate resistant acid phosphatase (TRAP) staining and cathepsin K immunohistochemistry. On day 3, the number of TRAP- and cathepsin K-positive cells increased more so in the PD group than in the C group. The PD+infliximab group showed a lower number of positive cells than the PD group. There was no difference in all the groups on day 20. On day 3, the cathepsin-K positive multinucleated and mononucleated cells were higher in the PD group than in the C group. The number of cathepsin-K positive multinucleated cells was lower in the PD+infliximab group than in the PD group. The PD group showed more cathepsin K-positive cells in the furcation and distal surfaces than the c group. The Cathepsin K-positive cells of the PD+infliximab group were lower than that of the PD group in furcation. These results suggest that TNF-α stimulates odontoclast formation in diabetes with periodontitis.

Keyword

TNF-α; odontoclasts; diabetes; periodontiti

MeSH Terms

Acid Phosphatase
Administration, Intravenous
Animals
Cathepsin K
Cathepsins
Immunohistochemistry
Infliximab
Ligation
Molar
Necrosis
Osteoclasts*
Periodontitis*
Rats*
Streptozocin
Acid Phosphatase
Cathepsin K
Cathepsins
Infliximab
Streptozocin
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