Osong Public Health Res Perspect.  2017 Aug;8(4):260-263. 10.24171/j.phrp.2017.8.4.05.

Aflatoxin M1 Contamination Levels in Cheeses Sold in Isfahan Province, Iran

Affiliations
  • 1Department of Microbiology, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
  • 2Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran. payamghasemidehkordi@yahoo.com
  • 3Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
  • 4Deputy of Research and Technology, Shahrekord University of Medical Sciences, Shahrekord, Iran.
  • 5Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran.

Abstract


OBJECTIVES
Aflatoxin M1 (AFM1)-contaminated dairy products pose serious human health risks, causing liver and renal failure if consumed. They are also related to decreased milk and egg production in infected animals. This study investigated the AFM1 contamination levels in cheeses sold in Isfahan province, Iran, by enzyme-linked immunosorbent assay (ELISA).
METHODS
A total of 100 white cheese samples were randomly collected from supermarkets in Isfahan province and after extraction using dichloromethane were prepared for the ELISA.
RESULTS
Of the 100 samples, 52 (52%) were contaminated by AFM1, at levels ranging from 50.2 to 424.4 ng/kg. The remaining 48% of the samples had undetectable AFM1 levels (< 50 ng/kg). Based on the standard limit set by the European Commission and Iran, 8% (8/100) of the AFM1-positive samples (with concentrations between 250.2 and 424.4 ng/kg) had levels higher than the permissible value of 250 ng/kg.
CONCLUSION
Although the percentage of cheese samples in Isfahan province with AFM1 levels exceeding the national permissible limit was low, the examination of cheeses and the milk used for their production is nevertheless important for ensuring public health. Furthermore, optimum storage conditions of animal feed should be ensured, and livestock nutrition must be monitored for the presence of AFM1 and other aflatoxins.

Keyword

aflatoxin M1; cheese; enzyme-linked immunosorbent assay

MeSH Terms

Aflatoxin M1*
Aflatoxins*
Animal Feed
Animals
Cheese*
Dairy Products
Enzyme-Linked Immunosorbent Assay
Humans
Iran*
Liver
Livestock
Methylene Chloride
Milk
Ovum
Public Health
Renal Insufficiency
Aflatoxin M1
Aflatoxins
Methylene Chloride
Full Text Links
  • OPHRP
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error