Lab Anim Res.  2011 Mar;27(1):41-46.

Mapping of the Faded (fe) Gene to a Region between D10mit191 and D10mit44 on Mouse Chromosome 10

• Oh SH
• Nam Y
• Suh JG

Abstract

The faded mouse is a coat color mutant that shows faded coat color and age-related loss of pigmentation. This mutation is transmitted by an autosomal recessive gene with 100% penetrance. In the present study, we carried out linkage analysis of the faded (fe) gene using intra-specific backcross panels. Affected faded mice were carefully confirmed by their faded coat color at about 4 weeks of age. In the intra-specific backcross between faded and CBA mice (n=198), the fe gene was mapped to a region 2.1 cM distal to D10mit191. Therefore, the gene order was defined as follows: centromere-D10mit51 (12.4+/-2.4 cM)-D10mit191 (2.1+/-1.0 cM)-fe-D10mit44 (13.3+/-2.4 cM)-D10mit42 (14.4+/-2.5 cM). This linkage map of the fe locus will provide a good entry point to isolate the fe gene. Since the faded mouse has pigmentary abnormalities, this mutant may be a useful model for studies of pigmentary abnormalities in humans.

Keyword

Genetic mapping; linkage analysis; faded gene; chromosome 10

MeSH Terms

Animals
Chromosomes, Human, Pair 10
Gene Order
Genes, Recessive
Humans
Mice
Mice, Inbred CBA
Penetrance
Pigmentation

Figure

• 

  Figure 1 Age-related pigment change of affected N2 progenies. A: 1 week, B: 2 weeks, C: 4 weeks, D: 28 weeks.

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  Figure 2 Haplotype analysis of an intraspecific backcross between fe and CBA/J mice [(C57BL/6J-fe/fe×CBA/J)F1×C57BL/6J-fe/fe]. Each column represents a chromosomal haplotype identified in the backcross and inherited from the F1 parent. The number of individuals is listed beneath each column. The black boxes represent fe alleles, and the white boxes represent CBA/J alleles as determined by SSLP scoring with markers indicated at the left.

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  Figure 3 Genetic linkage maps of chromosome 10 as derived from an intra backcross panel [(C57BL/6J-fe/fe×CBA/J)F1×C57BL/6J-fe/fe] generated in this study. Distances between intervals are shown in centimorgans±standard error to the left of the map.

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