Lab Anim Res.
2017 Sep;33(3):280-282. 10.5625/lar.2017.33.3.280.
Super pregnancy in a BALB/c mouse superovulated with PMSG
- Affiliations
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- 1Department of Clinical sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. mogheiseh@yahoo.com
- 2Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
- KMID: 2391103
- DOI: http://doi.org/10.5625/lar.2017.33.3.280
Abstract
- This paper reports a case of super pregnancy in a BALB/c mouse pregnant with 30 pups following induction of superovulation using a PMSG-hCG protocol. Superovulation was induced in 10 mice by injecting 5 IU PMSG followed by 5 IU hCG 48 hours later. Immediately after injection of hCG, animals were placed with males at a ratio of 1 to 1 for 24 hours. On day 14 after mating, animals were killed by cervical dislocation and the uterus was examined for pregnancy and the number of fetuses. The mean (±SEM) number of fetuses observed in ten mice was 5.4±3.18 with an unexpectedly super pregnant mouse bearing 30 fetuses on day 14 of pregnancy.
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