Lab Anim Res.  2017 Sep;33(3):209-215. 10.5625/lar.2017.33.3.209.

Artemisia argyi attenuates airway inflammation in lipopolysaccharide induced acute lung injury model

• Shin NR
• Park SH
• Ko JW
• Ryu HW
• Jeong SH
• Kim JC
• Shin DH
• Lee HS
• Shin IS

• KMID: 2391093
• DOI: http://doi.org/10.5625/lar.2017.33.3.209

Abstract

Artemisia argyi is used as a health supplement, tea, and food source in Korea. This study aimed to evaluate the effect of Artemisia argyi (AA) and its active compound, dehydromatricarin A (DA), on the attenuation of airway inflammation in a murine model of lipopolysaccharide (LPS)-induced acute lung injury (ALI). The C57BL/6 mice were administered AA (50 mg/kg or 100 mg/kg) and DA (10 mg/kg or 20 mg/kg) by oral gavage from day 0 to 7 days and LPS treated by intranasal instillation 48 hours before the sacrifice. The treatment of AA and DA markedly decreased inflammatory cells in the bronchoalveolar lavage fluid (BALF) compared with that in ALI-induced mice, which was accompanied by a significant reduction in the levels of tumor necrosis factor (TNF)-α and interleukin (IL)-6 in BALF. Furthermore, the administration of AA and DA clearly decreased inducible nitric oxide synthase (iNOS) expression and nuclear factor kappa B (NF-κB) phosphorylation in comparison with that in the ALI-induced mice. The histological examination of the lung tissue revealed that the administration of AA and DA suppressed the inflammatory cell infiltration into the peribronchial and alveolar lesions induced by LPS instillation. Collectively, our results indicated that AA and DA effectively decreased the airway inflammatory response induced by LPS instillation. Therefore, AA and DA may offer a potential therapy for airway inflammatory disease.

Keyword

Artemisia argyi; dehydromatricarin A; acute lung injury; inducible nitric oxide synthase; nuclear factor kappa B

MeSH Terms

Acute Lung Injury*
Animals
Artemisia*
Bronchoalveolar Lavage Fluid
Inflammation*
Interleukins
Korea
Lung
Mice
NF-kappa B
Nitric Oxide Synthase Type II
Phosphorylation
Tea
Tumor Necrosis Factor-alpha
Interleukins
NF-kappa B
Nitric Oxide Synthase Type II
Tea
Tumor Necrosis Factor-alpha

Figure

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  Figure 1 In BALF, AA and DA decreased inflammatory cell counts. NC, non-treated mice; LPS, LPS-induced mice; ROF, roflumilast 10 mg/kg per day and LPS-induced mice; AA-50, A. argyi 50 mg/kg per day and LPS-induced mice; AA-100, A. argyi 100 mg/kg per day and LPS-induced mice; DA-10, dehydromatricarin A 10 mg/kg per day and LPS-induced mice; DA-20, dehydromatricarin A 20 mg/kg per day and LPS-induced mice. The values shown are the mean±SD. #P<0.05 vs NC; *P<0.05 vs LPS.

• 

  Figure 2 AA and DA reduced the production of inflammatory cytokines. (A) TNF-α, (B) IL-6. NC, non-treated mice; LPS, LPSinduced mice; ROF, roflumilast 10 mg/kg per day and LPS-induced mice; AA-50, A. argyi 50 mg/kg per day and LPS-induced mice; AA-100, A. argyi 100 mg/kg per day and LPS-induced mice; DA-10, dehydromatricarin A 10 mg/kg per day and LPS-induced mice; DA-20, dehydromatricarin A 20 mg/kg per day and LPS-induced mice. The values are shown are the mean ± SD. #P<0.05 vs NC; *P<0.05 vs LPS.

• 

  Figure 3 AA and DA decreased the expression of iNOS and phosphorylation of NF-κB. NC, non-treated mice; LPS, LPS-induced mice; ROF, roflumilast 10 mg/kg per day and LPS-induced mice; AA-50, A. argyi 50 mg/kg per day and LPS-induced mice; AA-100, A. argyi 100 mg/kg per day and LPS-induced mice; DA-10, dehydromatricarin A 10 mg/kg per day and LPS-induced mice; DA-20, dehydromatricarin A 20 mg/kg per day and LPS-induced mice. The values are shown as the mean±SD. #P<0.05 vs NC; *P<0.05 vs LPS.

• 

  Figure 4 AA and DA attenuated inflammatory cell infiltration. NC, non-treated mice; LPS, LPS-induced mice; ROF, roflumilast 10 mg/kg per day and LPS-induced mice; AA-50, A. argyi 50 mg/kg per day and LPS-induced mice; AA-100, A. argyi 100 mg/kg per day and LPS-induced mice; DA-10, dehydromatricarin A 10 mg/kg per day and LPS-induced mice; DA-20, dehydromatricarin A 20 mg/kg per day and LPS-induced mice.

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