Immune Netw.  2017 Aug;17(4):228-236. 10.4110/in.2017.17.4.228.

Yersinia enterocolitica Exploits Signal Crosstalk between Complement 5a Receptor and Toll-like Receptor 1/2 and 4 to Avoid the Bacterial Clearance in M cells

Affiliations
  • 1Department of Molecular Biology and Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju 54896, Korea. yongsuk@jbnu.ac.kr
  • 2Department of Bioactive Material Sciences and Research Center of Bioactive Materials, Chonbuk National University, Jeonju 54896, Korea.

Abstract

In the intestinal mucosal surface, microfold cells (M cells) are the representative gateway for the uptake of luminal antigens. At the same time, M cells are the primary infection site for pathogens invading mucosal surface for their infection. Although it is well recognized that many mucosal pathogens exploit the M cells for their infection, the mechanism to infect M cells utilized by pathogens is not clearly understood yet. In this study, we found that M cells expressing complement 5a (C5a) receptor (C5aR) also express Toll-like receptor (TLR) 1/2 and TLR4. Infection of Yersinia enterocolitica, an M cell-invading pathogen, synergistically regulated cyclic adenosine monophosphate-dependent protein kinase A (cAMP-PKA) signaling which are involved in signal crosstalk between C5aR and TLRs. In addition, Y. enterocolitica infection into M cells was enhanced by C5a treatment and this enhancement was abrogated by C5a antagonist treatment. Finally, Y. enterocolitica infection into M cells was unsuccessful in C5aR knock-out mice. Collectively, we suggest that exploit the crosstalk between C5aR and TLR signaling is one of infection mechanisms utilized by mucosal pathogens to infect M cells.

Keyword

Complement 5a receptor; Immune evade; M cell; Toll-like receptor; Yersinia enterocolitica

MeSH Terms

Adenosine
Animals
Complement C5a*
Complement System Proteins*
Cyclic AMP-Dependent Protein Kinases
Mice
Mice, Knockout
Phenobarbital
Receptor, Anaphylatoxin C5a*
Toll-Like Receptors*
Yersinia enterocolitica*
Yersinia*
Adenosine
Complement C5a
Complement System Proteins
Cyclic AMP-Dependent Protein Kinases
Phenobarbital
Receptor, Anaphylatoxin C5a
Toll-Like Receptors
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