Ann Surg Treat Res.  2017 Jun;92(6):389-395. 10.4174/astr.2017.92.6.389.

A simple rat model of in situ reversible obstructive jaundice in situ reversible obstructive jaundice model

• Huang X
• Li CH
• Zhang AQ
• Kong Z
• Gu WQ
• Dong JH

• KMID: 2387411
• DOI: http://doi.org/10.4174/astr.2017.92.6.389

Abstract

PURPOSE
To develop a simple and reliable rat model of in situ reversible obstructive jaundice with low morbidity and mortality rates.
METHODS
Rats were divided into 4 groups with 8 rats each: the sham-operated (SH) group only underwent laparotomy, the control internal drainage (ID-C) group underwent choledochoduodenostomy, the new internal drainage (ID-N) group and the long-term internal drainage (ID-L) group underwent choledochocholedochostomy. Common bile duct ligation was performed in all the drainage groups 7 days before reversal procedures. All rats were sacrificed for samples 7 days after the last operation except rats of the ID-L group that survived 28 days before sacrifice. Body weight, liver function, histopathological changes, morbidity and mortality were assessed.
RESULTS
One rat died and 2 rats had complications with tube blockage in the ID-C group. No death or complications occurred in the ID-N and ID-L groups. The drainage tube remained patent in the long-term observation ID-L group. Body weight showed no significant difference between the ID-C and ID-N groups after 7 days drainage. Liver function was not fully recovered in the ID-C and ID-N groups after 7 days drainage, but statistical differences were only observed in the ID-C group compared with the SH and ID-L groups. Periportal inflammation and bile duct proliferation showed severer in the ID-C group than in the ID-N group.
CONCLUSION
The present study provided an efficient, simple, and reliable rat model that is especially suitable for long-term or consecutive studies of reversible obstructive jaundice.

Keyword

Animal model; Drainage; Obstructive jaundice; Rats

MeSH Terms

Animals
Bile Ducts
Body Weight
Choledochostomy
Common Bile Duct
Drainage
Inflammation
Jaundice, Obstructive*
Laparotomy
Ligation
Liver
Models, Animal*
Mortality
Rats*

Figure

• 

  Fig. 1 Surgical procedure of inducing obstructive jaundice (OJ). (A) Expose the common bile duct (CBD) without pulling out the duodenum. The arrow showed the whole CBD. (B) Double ligation and transection of the CBD for inducing OJ. The white arrow showed the hepatic side of the CBD. The black arrow showed the pancreatic side of the CBD.

• 

  Fig. 2 Surgical procedure of releasing obstructive jaundice (OJ) in situ. (A) Operating field before the reversal procedure. The arrow showed the pancreatic side of the CBD. (B) A plastic tube was inserted into the pancreatic side of the CBD. (C) Operating field after the second operation. The 2 sides of the CBD were connected with the tube.

• 

  Fig. 3 Body weight changes in the 4 groups. ID-C, control internal drainage group; ID-L, long-term internal drainage group; ID-N, new internal drainage group; SH, shamoperated group. *P < 0.05, 14 days vs. 7 days in ID-C and ID-N, respectively. **P < 0.01, SH vs. ID-C, ID-N, and ID-L, respectively. ***P < 0.001. 35 days vs. 7 days in ID-L.

• 

  Fig. 4 Morphologic changes of the liver in the 4 groups. (A) Normal liver of the SH group (H&E, ×200). (B) Recovered portal area of the ID-L group (H&E, ×200). (C) Moderate periportal inflammation and bile duct proliferation of the ID-C group (H&E, ×100). (D) Mild periportal inflammation and bile duct proliferation of the ID-N group (H&E, ×100). ID-C, control internal drainage group; ID-L, longterm internal drainage group; ID-N, new internal drainage group.

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