Korean J Parasitol.  2017 Jun;55(3):341-345. 10.3347/kjp.2017.55.3.341.

Artyfechinostomum malayanum: Metacercariae Encysted in Pila sp. Snails Purchased from Phnom Penh, Cambodia

Affiliations
  • 1Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 52727, Korea. wmshon@gnu.ac.kr
  • 2Department of Environmental Medical Biology and Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 03722, Korea.
  • 3Department of Parasitology, Medical Research Institute and Parasite Resource Bank, School of Medicine, Chungbuk National University, Cheongju 28644, Korea.
  • 4Center for National Malaria Control, Phnom Penh, Cambodia.
  • 5Institute of Parasitic Diseases, Korea Association of Health Promotion, Seoul 07653, Korea. cjy@snu.ac.kr
  • 6Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 03080, Korea.

Abstract

The metacercariae of Artyfechinostomum malayanum (Leiper, 1911) Mendheim, 1943 were discovered in Pila sp. snails purchased from a market in Phnom Penh, Cambodia. They were isolated from the snails using the artificial digestion technique and were orally fed to 2 hamsters, 1 rat, and 2 mice to obtain the adult flukes. The metacercariae were round, 145–165 μm in diameter, having a cyst wall of 6–10 μm in thickness, a head collar and collar spines, and characteristic features of excretory granules. Adult flukes were recovered in the small intestines of the animals at days 14 and 32 post infection and were morphologically observed using a light microscope and a scanning electron microscope. They were plump or elongated, ventrally curved, 6.0–8.1×1.6–2.0 mm in size, and characterized by the head collar bearing 43 collar spines, including 5 end group ones on each side, a long cirrus sac extending beyond the posterior margin of the ventral sucker, a submedian ovary, and 2 deeply lobed testes. Eggs in uteri were operculate, ovoid to ellipsoid, and 120–135×68–75 μm in size. In scanning electron microscopy, the head collar was prominent with collar spines looking like horns. Scale-like tegumental spines were densely distributed on the ventral surface between the head collar and ventral sucker. Sensory papillae were distributed mainly on the tegument around suckers. By this study, it has been first confirmed that the life cycle of A. malayanum exists in Cambodia.

Keyword

Artyfechinostomum malayanum; echinostome; metacercaria; adult fluke; Pila sp. snail; scanning electron microscopy; Cambodia
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