Nat Prod Sci.  2017 Jun;23(2):139-145. 10.20307/nps.2017.23.2.139.

Flavonoid Compounds from the Leaves of Kalanchoe prolifera and Their Cytotoxic Activity against P-388 Murine Leukimia Cells

Affiliations
  • 1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Jenderal Achmad Yani, University, Cimahi 40528, Indonesia.
  • 2Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia. unang.supratman@unpad.ac.id
  • 3Central Laboratory of Universitas Padjadjaran, Jatinangor 45363, Indonesia.
  • 4Department of Food, Life, and Environmental Science, Faculty of Agriculture, Yamagata University, Tsuruoka, Yamagata 997-8555, Japan.

Abstract

Seven flavonoid compounds, kaempferol (1), quercetin (2), quercetin-3-O-β-D-glucopyranoside (3), kaempferol-3-O-β-D-glucopyranoside (4), kaempferol-3-O-α-L-rhamnoside (5), quercetin-3-O-sophoroside (6) and quercetin-3-O-rutinoside (7), were isolated from the methanolic extract of leaves of Kalanchoe prolifera. Compounds 1-7 were isolated for first time from this plant. These compounds were evaluated their cytotoxic activity against P-388 murine leukimia cells in vitro. Among those compounds kaempferol (1) and quercetin (2) showed strongest cytotoxic activity with ICâ‚…â‚€ values of 4.45 ± 0.05 and 6.28 ± 0.02 µg/mL, respectively.

Keyword

Flavonoids; Kalanchoe prolifera; Crassulaceae; P-388 murine leukimia cell

MeSH Terms

Crassulaceae
Flavonoids
In Vitro Techniques
Kalanchoe*
Methanol
Plants
Quercetin
Flavonoids
Methanol
Quercetin

Figure

  • Fig. 1. The structure of compounds 1 – 7 isolated from K. prolifera.

  • Fig. 2. Key HMBC (→) and COSY () correlations of 3.


Reference

References

(1). Heyne K.The useful Indonesian plants: Research and Development Agency; Ministry of Forestry: Indonesia,. 1987. 845–846.
(2). Hutapea J. R.Inventory of Indonesian Medicinal Plants Vol: Research and Development Agency; Ministry of Health: Indonesia,. 1994. 117–118.
(3). Capon R. J.., Macleod J. K.., Oelrichs P. B.Aust. J. Chem,. 1986. 39:1711–1715.
(4). Yamagishi T.., Haruna M.., Yan X. Z.., Chang J. J.., Lee K. H. J.Nat. Prod,. 1989. 52:1071–1079.
(5). Supratman U.., Fujita T.., Akiyama K.., Hayashi H.Biosci. Biotechnol. Biochem. 2000. 64:1310–1312.
(6). Gaind K. N.., Singla A. K.., Boar R. B.., Copsey D. B.Phytochemistry. 1976. 15:1999–2000.
(7). Costa S. S.., Jossang A.., Bodo B. J.Nat. Prod. 1996. 59:327–329.
(8). Liu K. C. S.., Yang S. L.., Robert M. F.., Phillipson J. D.Phytochemistry. 1989. 28:2813–2818.
(9). Liu K. C. S.., Yang S. L.., Robert M. F.., Phillipson J. D. J.Nat. Prod. 1989. 52:970–974.
(10). Almeida A. P.., Da Silva S. A.., Souza M. L. M.., Lima L. M.., Rossi-Bergmann B.., de Moraes V. L.., Costa S. S.Planta Med. 2000. 66:134–137.
(11). Wu P. L.., Hsu Y. L.., Wu T. S.., Bastow K. F.., Lee K. H.Org. Lett. 2006. 8:5207–5210.
(12). Supratman U.., Fujita T.., Akiyama K.., Hayashi H.Phytochemistry. 2001. 58:311–314.
(13). Supratman U.., Fujita T.., Akiyama K.., Hayashi H.., Murakami A.., Sakai H.., Koshimizu K.., Ohigashi H.Biosci. Biotechnol. Biochem. 2001. 65:947–949.
(14). Toker G.., Memisoglu M.., Yesilada E.., Aslan M.Turk. J. Chem,. 2004. 28:745–749.
(15). Markam H. M.Techiques of Flavonoid Identification. Academic Press: London;1982. p. 41–47.
(16). Guvenalp Z.., Demirezer L. O.Turk. J. Chem. 2005. 29:163–169.
(17). Chaturvedula V. S. P.., Prakash I. J.Chem. Pharm. Res.,. 2011. 3:799–804.
(18). Kim J. W.., Kim T. B.., Yang H.., Sung S. H.Nat. Prod. Sci. 2016. 22:117–121.
(19). Lin L. J.., Huang X. B.., Lv Z. C.Springerplus,. 2016. 5:1649.
(20). Aisyah L. S.., Yun Y. F.., Julaeha E.., Herlina T.., Zainuddin A.., Hermawan W.., Supratman U.., Hayashi H.Open Chemistry Journal. 2015. 2:36–39.
(21). Islam M.., Al-Amin Md.., Siddiqi M. M. A.., Akter S.., Haque M. M.., Sultana N.., Chowdhury A. M. S.DhakaUniv. J. Sci. 2012. 60:11–14.
(22). Si C. L.., Du Z. G.., Fan S.., An L. L.Chem. Nat. Comp. 2016. 52:794–797.
(23). Furusawa M.., Tanaka T.., Ito T.., Nakaya K.., Iliya I.., Ohyama M.., Iinuma M.., Murata H.., Inatomi Y.., Inada A.., Nakanishi T.., Matsushita S.., Kubota Y.., Sawa R.., Takahashi Y.Chem. Pharm. Bull. 2005. 53:591–593.
(24). Ross S. A.., ElSohly M. A.., Sultana G. N.., Mehmedic Z.., Hossain C. F.., Chandra S.Pyhtochem. Anal. 2005. 16:45–48.
(25). Li H.., Liu Y.., Yi Y.., Miao Q.., Liu S.., Zhao F.., Cong W.., Wang C.., Xia C. J.Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2017. 1048:56–63.
(26). Zhang S.., Tao Z. M.., Zhang Y.., Shen Z. W.., Qin G. W.Chin. J. Nat. Med. 2010. 8:21–24.
(27). Ganbaatar C.., Gruner M.., Mishig D.., Duger R.., Schmidt A. W.., Knölker H. J.The Open Natural Products Journal. 2015. 8:1–7.
(28). Alley M. C.., Scudiero D. A.., Monks A.., Hursey M. L.., Czerwinski M. J.., Fine D. L.., Abbott B. J.., Mayo J. G.., Shoemaker R. H.., Boyd M. R.Cancer Res. 1988. 48:589–601.
(29). Hakim E. H.., Juliawaty L. D.., Syah Y. M.., Din L. B.., Ghisalberti E. L.., Latip J.., Said I. M.., Achmad S. A. Z.Naturforsch. C. 2005. 60:723–727.
(30). Harneti D.., Tjokronegoro R.., Safari A.., Supratman U.., Loong X. M.., Mukhtar M. R.., Mohamad K.., Awang K.., Hayashi H.Phytochem. Lett. 2012. 5:496–499.
(31). Hakim E. H.., Achmad S. A.., Juliawaty L. D.., Makmur L.., Syah Y. M.., Aimi N.., Kitajima M.., Takayama H.., Ghisalberti E. L. J.Nat. Med. 2007. 61:229–236.
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