Nat Prod Sci.  2017 Jun;23(2):92-96. 10.20307/nps.2017.23.2.92.

Inhibitory Effect of a Sesquiterpene from Artemisia iwayomogi on Expression of Inducible Nitric Oxide Synthase by Suppression of I-κBα Degradation in LPS-stimulated RAW 264.7 Cells

Affiliations
  • 1College of Pharmacy, Sookmyung Women's University, Seoul 04310, Republic of Korea. ryuha@sookmyung.ac.kr
  • 2Department of Natural Medicine Resources, Semyung University, Jecheon, Chungbuk 27136, Republic of Korea. hwalee@semyung.ac.kr

Abstract

A sesquiterpene was purified from Artemisia iwayomogi methanolic extract during the course of searching anti-inflammatory principle from medicinal plants. A sesquiterpene identified as armefolin inhibited the production of nitric oxide (NO) and attenuated inducible nitric oxide synthase (iNOS) protein level in lipopolysaccharide (LPS)-activated RAW 264.7 cells. Armefolin also down-regulated mRNA expressions of iNOS and pro-inflammatory cytokines, interleukin-1β and interleukin-6 in LPS-activated macrophages. Moreover, armefolin suppressed the degradation of inhibitory-κBα (I-κBα) in LPS-activated macrophages. These data suggest that armefolin from A. iwayomogi can suppress the LPS-induced production of NO and the expression of iNOS gene through inhibiting the degradation of I-κBα. Taken together, armefolin from A. iwayomogi might be a candidate as promising anti-inflammatory agent.

Keyword

Artemisia iwayomogi; Armefolin; Sesquiterpene; Nitric oxide; Inducible nitric oxide synthase

MeSH Terms

Artemisia*
Cytokines
Interleukin-6
Macrophages
Methanol
Nitric Oxide
Nitric Oxide Synthase Type II*
Plants, Medicinal
RAW 264.7 Cells*
RNA, Messenger
Cytokines
Interleukin-6
Methanol
Nitric Oxide
Nitric Oxide Synthase Type II
RNA, Messenger
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