Biomol Ther.  2017 Jul;25(4):367-373. 10.4062/biomolther.2016.174.

Circulating Plasma and Exosomal microRNAs as Indicators of Drug-Induced Organ Injury in Rodent Models

Affiliations
  • 1Department of Molecular Medicine, Kyungpook National University, Daegu 41944, Republic of Korea. mcbaek@knu.ac.kr
  • 2Department of Pharmacology, Kyungpook National University, Daegu 41944, Republic of Korea.
  • 3Department of Biochemistry and Cell Biology, CMRI, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
  • 4Section of Molecular Pharmacology and Toxicology, Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, NIH, Bethesda, MD 20892, USA.

Abstract

This study was performed to evaluate whether microRNAs (miRNAs) in circulating exosomes may serve as biomarkers of drug-induced liver, kidney, or muscle-injury. Quantitative PCR analyses were performed to measure the amounts of liver-specific miRNAs (miR-122, miR-192, and miR-155), kidney-specific miR-146a, or muscle-specific miR-206 in plasma and exosomes from mice treated with liver, kidney or muscle toxicants. The levels of liver-specific miRNAs in circulating plasma and exosomes were elevated in acetaminophen-induced liver injury and returned to basal levels by treatment with antioxidant N-acetyl-cysteine. Circulating miR-146a and miR-206 were increased in cisplatin-induced nephrotoxicity and bupivacaine-induced myotoxicity, respectively. Taken together, these results indicate that circulating plasma and exosomal miRNAs can be used as potential biomarkers specific for drug-induced liver, kidney or muscle injury.

Keyword

miRNAs; Exosomes; Liver-specific injury; N-acetyl cysteine; Biomarkers

MeSH Terms

Animals
Biomarkers
Exosomes
Kidney
Liver
Mice
MicroRNAs*
Plasma*
Polymerase Chain Reaction
Rodentia*
Biomarkers
MicroRNAs
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