Yonsei Med J.  2014 Mar;55(2):428-434.

Single Nucleotide Polymorphisms of Toll-Like Receptor 7 and Toll-Like Receptor 9 in Hepatitis C Virus Infection Patients from Central China

• Wei XS
• Wei CD
• Tong YQ
• Zhu CL
• Zhang PA

Abstract

PURPOSE
To analyze the correlation of polymorphisms of toll-like receptor 7 (TLR7) (rs179009) and toll-like receptor 9 (TLR9) (rs187084) in hepatitis C virus (HCV) infections in the Han population.
MATERIALS AND METHODS
The genotypes of TLR7IVS2-151 in HCV infection were detected by Sanger sequencing using polymerase chain reaction-restriction fragment length polymorphism to determine the TLR9 T-1486C single nucleotide polymorphisms (SNP) for all enrolled patients.
RESULTS
We found no significant difference between males with spontaneous clearance of HCV versus those chronically infected [chi2=2.71, p=0.10, odd ratios (OR)=0.58, 95% confidence interval (CI) 0.31-1.11]. However, significant differences were found for the distribution of TLR7 (rs179009) in females (chi2=9.46, p=0.01). In females, a significant difference was also found between chronic hepatitis C and those with spontaneous clearance of HCV in terms of TLR7 IVS2-151G/A allele frequencies (chi2=9.50, p=0.00, OR=0.46, 95% CI 0.28-0.75). In HCV-infected patients, no significant association was found between the frequency of TLR9 genotypes and alleles.
CONCLUSION
The site of TLR7 IVS2-151 (rs179009) G/A may be a factor for susceptibility of chronic HCV in the female Han population. TLR9T-1486C (rs18084) SNP may not play a major role in HCV infection. However, individual risk profiles for HCV infection did vary by sex and this relationship should be further investigated.

Keyword

Hepatitis C virus; single nucleotide polymorphism; TLR7; TLR9

MeSH Terms

Alleles
China*
Confidence Intervals
Female
Gene Frequency
Genotype
Hepacivirus*
Hepatitis C*
Hepatitis C, Chronic
Hepatitis*
Humans
Male
Methods
Polymorphism, Single Nucleotide*
Toll-Like Receptor 7*
Toll-Like Receptor 9*
Toll-Like Receptors*
Toll-Like Receptor 7
Toll-Like Receptor 9
Toll-Like Receptors

Figure

• 

  Fig. 1 Genotyping of the TLR7 gene polymorphism by singer sequencing. (A) The genotype is AA. (B) The genotype is GA. (C) The genotype is GA. TLR, toll-like receptor.

• 

  Fig. 2 Genotyping of the TLR9. M means DNA maker, lane 1 CT are digested in to three fragments, lane 2 CC are undigested, lane 3 digested into two fragments. TLR, toll-like receptor.

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