Korean J Thorac Cardiovasc Surg.  2017 Apr;50(2):126-129. 10.5090/kjtcs.2017.50.2.126.

Circulating Aneuploid Cells Detected in the Blood of Patients with Infectious Lung Diseases

Affiliations
  • 1Department of Thoracic and Cardiovascular Surgery, Sungkyunkwan University School of Medicine, Korea. jkimsmc@skku.edu
  • 2Samsung Biomedical Research Institute, Samsung Medical Center, Sungkyunkwan University School of Medicine, Korea.

Abstract

The identification of circulating tumor cells (CTCs) is clinically important for diagnosing cancer. We have previously developed a size-based filtration platform followed by epithelial cell adhesion molecule immunofluorescence staining for detecting CTCs. To characterize CTCs independently of cell surface protein expression, we incorporated a chromosomal fluorescence in situ hybridization (FISH) assay to detect abnormal copy numbers of chromosomes in cells collected from peripheral blood samples by the size-based filtration platform. Aneuploid cells were detected in the peripheral blood of patients with lung cancer. Unexpectedly, aneuploid cells were also detected in the control group, which consisted of peripheral blood samples from patients with benign lung diseases, such as empyema necessitatis and non-tuberculous mycobacterial lung disease. These findings suggest that chromosomal abnormalities are observed not only in tumor cells, but also in benign infectious diseases. Thus, our findings present new considerations and bring into light the possibility of false positives when using FISH for cancer diagnosis.

Keyword

Circulating neoplastic cells; Fluorescent in situ hybridization; Lung neoplasms

MeSH Terms

Aneuploidy*
Chromosome Aberrations
Communicable Diseases
Diagnosis
Empyema
Epithelial Cells
Filtration
Fluorescence
Fluorescent Antibody Technique
Humans
In Situ Hybridization
In Situ Hybridization, Fluorescence
Lung Diseases*
Lung Neoplasms
Lung*
Neoplastic Cells, Circulating
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