Biomol Ther.  2015 Jan;23(1):90-97. 10.4062/biomolther.2014.080.

Anti-Inflammatory Effects of Water Chestnut Extract on Cytokine Responses via Nuclear Factor-kappaB-signaling Pathway

• Kim B
• Kim JE
• Choi BK
• Kim HS

• KMID: 2380368
• DOI: http://doi.org/10.4062/biomolther.2014.080

Abstract

Water chestnut (Trapa japonica Flerov.) is an annual aquatic plant. In the present study, we showed that the treatment of water chestnut extracted with boiling water resulted in a significant increase 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and decrease the intracellular H2O2-induced accumulation of reactive oxygen species. In addition, water chestnut extract (WCE) inhibited lipopolysaccharide (LPS)-induced nitric oxide production and suppressed mRNA and protein expression of the inducible nitric oxide synthase gene. The cytokine array results showed that WCE inhibited inflammatory cytokine secretion. Also, WCE reduced tumor necrosis factor-alpha- and interleukin-6-induced nuclear factor-kappaB activity. Furthermore, during sodium lauryl sulfate (SLS)-induced irritation of human skin, WCE reduced SLS-induced skin erythema and improved barrier regeneration. These results indicate that WCE may be a promising topical anti-inflammatory agent.

Keyword

Trapa japonica Flerov.; Water chestnut; Anti-inflammatory effect; Cytokine; NF-kappaB

MeSH Terms

Eleocharis*
Erythema
Humans
Necrosis
NF-kappa B
Nitric Oxide
Nitric Oxide Synthase Type II
Plants
Reactive Oxygen Species
Regeneration
RNA, Messenger
Skin
Sodium Dodecyl Sulfate
NF-kappa B
Nitric Oxide
Nitric Oxide Synthase Type II
RNA, Messenger
Reactive Oxygen Species
Sodium Dodecyl Sulfate