J Korean Med Sci.  2017 Jul;32(7):1118-1123. 10.3346/jkms.2017.32.7.1118.

Significance of Serology by Multi-Antigen ELISA for Tissue Helminthiases in Korea

• Jin Y
• Kim EM
• Choi MH
• Oh MD
• Hong ST

• KMID: 2379606
• DOI: http://doi.org/10.3346/jkms.2017.32.7.1118

Abstract

It is clinically important to differentiate tissue-invading helminthiasis. The purpose of this study was to assess the specific immunoglobulin G (IgG) antibody positive rates for clonorchiasis, paragonimiasis, cysticercosis, and sparganosis 4 helminthiases from 1996 to 2006 using multi-antigen enzyme-linked immunosorbent assay (ELISA) in Korea. Results of 6,017 samples, which were referred to our institute for serodiagnosis, were analyzed. The subjects with positive serum IgG antibodies were 1,502 (25.0%) for any of the 4 helminthiases. The overall positive numbers for clonorchiasis, paragonimiasis, cysticercosis, and sparganosis were 728 (12.1%), 166 (2.8%), 729 (12.1%), and 263 (4.4%), respectively. The positive serologic reaction to multi-antigens was determined in 309 (20.6%) of the 1,502 total seropositive subjects. Those with multi-antigen positivity were regarded as positive for the antigen of strongest reaction but cross-reaction to others with weak positive reaction. Annual seropositive rates for those 4 tissue helminthiases ranged from 12.1% to 35.7%. The highest rate was observed in age from 60 to 69 years old and prevalence of men (27.4%; 1,030/3,763) was significantly higher than of women (19.1%; 332/1,741). Hospital records of 165 ELISA positive patients were reviewed to confirm correlation with their clinical diagnosis. Paragonimiasis was highly correlated as 81.8% (9/11), cysticercosis 29.9% (20/67), clonorchiasis 29.0% (20/69), and sparganosis 11.1% (2/18). In conclusion, the multi-antigen ELISA using 4 helminth antigens is useful to differentiate suspected tissue-invading helminthiases, especially ELISA diagnosis of paragonimiasis is reliable. The seropositivity is still high among suspected patients in Korea.

Keyword

Tissue Helminthiases; Seropositivity; IgG Antibody; ELISA; Cross-Reaction; Multiple Infections; Korea

MeSH Terms

Antibodies
Antigens, Helminth
Clonorchiasis
Cysticercosis
Diagnosis
Enzyme-Linked Immunosorbent Assay*
Female
Helminthiasis*
Hospital Records
Humans
Immunoglobulin G
Korea*
Male
Paragonimiasis
Prevalence
Serologic Tests
Sparganosis
Antibodies
Antigens, Helminth
Immunoglobulin G

Figure

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  Fig. 1 Changes in ELISA positive rates of clonorchiasis, paragonimiasis, sparganosis, and cysticercosis among suspected patients in Korea from 1996 to 2006.ELISA = enzyme-linked immunosorbent assay.

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  Fig. 2 Comparison of the single- and multi-positive cases in 4 helminthiases.

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  Fig. 3 ELISA absorbance distribution of clinically confirmed and suspected patients.ELISA = enzyme-linked immunosorbent assay, Cs = clonorchiasis, Pw = paragonimiasis, Sp = sparganosis, Cy = cysticercosis.*P = 0.009.

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  Fig. 4 ELISA absorbance of patients with clonorchiasis and clonorchiasis with cholangiocarcinoma.ELISA = enzyme-linked immunosorbent assay, Cs = clonorchiasis.

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