Korean J Physiol Pharmacol.  2017 May;21(3):335-343. 10.4196/kjpp.2017.21.3.335.

Therapeutic effects of orally administered CJLP55 for atopic dermatitis via the regulation of immune response

• Hyung KE
• Kim SJ
• Jang YW
• Lee DK
• Hyun KH
• Moon BS
• Kim B
• Ahn H
• Park SY
• Sohn UD
• Park ES
• Hwang KW

• KMID: 2376962
• DOI: http://doi.org/10.4196/kjpp.2017.21.3.335

Abstract

Atopic dermatitis (AD) is an inflammatory skin condition accompanied by symptoms such as edema and hemorrhage. Kimchi is a traditional fermented Korean dish consisting of various probiotics. In this study, the therapeutic effect of Lactobacillus plantarum CJLP55 isolated from Kimchi was studied in AD-induced mice. Orally administered Lactobacillus strain, CJLP55, suppressed AD symptoms and high serum IgE levels. CJLP55 administration reduced the thickness of the epidermis, infiltration of mast cells and eosinophils into the skin lesion, enlargement of axillary lymph nodes, and increase in cell population in axillary lymph nodes. CJLP55 treatment decreased the production of type 2 cytokines, such as interleukin (IL)-4, IL-5, IL-10, IL-12, interferon (IFN)-γ, and IL-6,which were stimulated by house dust mite extracts, in the axillary lymph node cells. Orally administered CJLP55 exhibited a therapeutic effect on house dust mite-induced AD in NC/Nga mice after onset of the disease by altering immune cell activation. The Lactobacillus strain, CJLP55, isolated from Kimchi, suppressed AD. Our results suggest its possible use as a potential candidate for management of AD.

Keyword

Atopic; Dermatitis; Dermatophagoides farinae; Lactobacillus; Probiotics; Th2 cells

MeSH Terms

Animals
Cytokines
Dermatitis
Dermatitis, Atopic*
Dermatophagoides farinae
Dust
Edema
Eosinophils
Epidermis
Hemorrhage
Immunoglobulin E
Interferons
Interleukin-10
Interleukin-12
Interleukin-5
Interleukins
Lactobacillus
Lactobacillus plantarum
Lymph Nodes
Mast Cells
Mice
Probiotics
Pyroglyphidae
Skin
Th2 Cells
Therapeutic Uses*
Cytokines
Dust
Immunoglobulin E
Interferons
Interleukin-10
Interleukin-12
Interleukin-5
Interleukins
Therapeutic Uses

Figure

• 

  Fig. 1 Experimental procedure and effects of orally administered CJLP55 on atopic dermatitis induced by house dust mite extract.(a) Experimental procedure for induction of atopic dermatitis and administration of lactobacilli was illustrated. Day zero is defined as the first day of administration of CJLP55 strain. (b) The dermatitis scores were evaluated by observing erythema/hemorrhage, scarring/dryness, excoriation/erosion, and edema. The score was noted once every two weeks until day 56. (c) The photographs were obtained in week eight before the mice were sacrificed. The photographs of NC/Nga mice with Dfb-induced dermatitis were obtained on day 55. (d) The level of total IgE in collected serum on day 28 was determined by ELISA. Data are shown as mean±SD of changes in the dermatitis score and total IgE level of five mice (n=5). *p<0.05; **p<0.01; ***p<0.001 compared with control.

• 

  Fig. 2 Effect of orally administered CJLP55 on the thickness of the dorsal skin.(a) Histological analysis of the skin lesion after H&E staining. This is a representative image of five mice. (b) Epidermal thickness of the dorsal skin was measured. Data are shown as mean±SD of changes in the skin thickness of five mice (n=5). *p<0.05; **p<0.01; ***p<0.001 compared with control.

• 

  Fig. 3 Effects of orally administered CJLP55 on infiltration of mast cells and eosinophils in the inflammatory site.The paraffin block of the dorsal skin was sectioned and stained with toluidine blue (a) and congo red (c) for mast cells and eosinophils, respectively. The number of mast cells (b) and eosinophils (d) were counted under a microscope. Data are shown as mean±SD of five mice. *p<0.05; **p<0.01; ***p<0.001 compared with control.

• 

  Fig. 4 Effects of orally administered CJLP55 on the axillary lymph nodes in NC/Nga mice.(a) Total cell number was measured from cell suspensions of the axillary lymph nodes. The number of T cells (b), B cells (c), macrophages (d), and dendritic cells (e) was analysed by flow cytometry. Data are shown as mean±SD of five mice. *p<0.05; **p<0.01; ***p<0.001 compared with control.

• 

  Fig. 5 Effects of orally administered CJLP55 on the cytokine production in the axillary lymph node cells.On day 56, axillary lymph node cells were isolated and cells were stimulated with 10 µgmL−1 of Dfb extract for 48 h. The concentration of IL-4 (a), IL-5 (b), IL-10 (c), IL-12 (d), IFN-γ (e), and IL-6 (f) was measured by ELISA. Data are shown as mean±SD of three independent experiments. *p<0.05; **p<0.01; ***p<0.001 compared with control.

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