Mycobiology.  2017 Mar;45(1):44-47. 10.5941/MYCO.2017.45.1.44.

Control Efficacy of Streptomyces sp. A501 against Ginseng Damping-off and Its Antifungal Substance

Affiliations
  • 1Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 54596, Korea. bsyun@jbnu.ac.kr
  • 2Division of Crop Science, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea.
  • 3Agricultural Microbiology Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA), Wanju 55365, Korea.
  • 4Ginseng Division, National Institute of Horticultural & Herbal Science, Rural Development Administration (RDA), Eumseong 27709, Korea.

Abstract

Ginseng damping-off, caused by the fungal pathogens Rhizoctonia solani and Pythium sp., is a critical disease in ginseng seedling. In a continuing effort to find microorganisms with the potential of acting as a biocontrol agent against Rhizoctonia damping-off, we found that a Streptomyces sp. A501 showed significant antifungal activity against Rhizoctonia solani. In field experiment to test the efficacy of Streptomyces sp. A501 in controlling ginseng damping-off, the incidence of damping-off disease was meaningfully reduced when ginseng seeds were soaked in the culture broth of Streptomyces sp. A501 before sowing. To perform characterization of the antifungal compound, we isolated it from the culture broth of strain A501 through Diaion HP-20 and silica gel column chromatographies and preparative high-performance liquid chromatography. The structure of the antifungal compound was assigned as fungichromin by spectroscopic methods, mainly nuclear magnetic resonance and electrospray ionization-mass analysis.

Keyword

Fungichromin; Ginseng damping-off; Rhizoctonia solani; Streptomyces sp. A501

MeSH Terms

Chromatography
Chromatography, Liquid
Incidence
Magnetic Resonance Spectroscopy
Panax*
Pythium
Rhizoctonia
Seedlings
Silica Gel
Streptomyces*
Silica Gel
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