J Korean Acad Oral Health.  2017 Mar;41(1):65-70. 10.11149/jkaoh.2017.41.1.65.

Antimicrobial effect of Prunus mume extracts against cariogenic bacteria

• Lee JS
• Chung KH

• KMID: 2376041
• DOI: http://doi.org/10.11149/jkaoh.2017.41.1.65

Abstract

OBJECTIVES
This study was conducted to determine whether Prunus mume extracts have an antimicrobial effect against Streptococcus mutans (S. mutans) and Streptococcus sobrinus (S. sobrinus).
METHODS
The study used crushed and dried Prunus mume, to which 80% methanol was added to obtain extracts. The extracts then underwent a demarcation process, sequentially using hexane, chloroform, and ethyl acetate, all of which have different polarities, followed by a reduction in pressure . The disc diffusion method was then used to measure the clear zone diameter to identify the antimicrobial effect of Prunus mume extracts using the different solvents. The methanol extracts that presented antimicrobial activity against S. mutans and S. sobrinus were then selected, and their optical densities (3, 6, 9, 12, and 24 h after cultivation) were measured to identify growth retardation effects based on extract concentration (0.01, 0.1, 1, and 5 mg/ml).
RESULTS
A clear zone was observed in methanol and ethyl acetate for S. mutans when the antimicrobial effect of Prunus mume extracts of each solvent against oral microorganisms was measured via the disc diffusion method. A clear zone was observed in hexane, chloroform, methanol, and ethyl acetate, when the extracts were tested for antimicrobial activity against S. sobrinus. The extract concentration of 1 mg/ml retarded growth with a statistical significance (P<0.05) from 6 h onwards, as determined when the optical density was measured hourly and the growth curves of S. mutans and S. sobrinus were plotted.
CONCLUSIONS
Prunus mume extracts retarded the growth of S. mutans and S. sobrinus with increase in time and concentration. Therefore, Prunus mume extracts hold the potential to be used for developing an oral antimicrobial agent to control dental caries.

Keyword

Antimicrobial effect; Prunus mume; Streptococcus mutans; Streptococcus sobrinus

MeSH Terms

Bacteria*
Chloroform
Dental Caries
Diffusion
Methanol
Methods
Prunus*
Solvents
Streptococcus mutans
Streptococcus sobrinus
Chloroform
Methanol
Solvents

Figure

• 

  Fig. 1 Growth curves of S. mutans cultured at different concentration of Prunus mume.

• 

  Fig. 2 Growth curves of S. sobrinus cultured at different concentration of Prunus mume.

Reference

1. Hamada S, Koga T, Ooshima T. Virulence factors of Streptococcus mutans and dental caries prevention. J Dent Res. 1984; 63:407–411.

2. Loesche WJ. Role of Streptococcus mutans in human dental decay. Microbiol Rev. 1986; 50:353–380.

3. Hamada S, Koga T, Ooshima T. Virulence factors of Streotococcus mutans and dental caries prevention. J Dent Res. 1984; 63:407–411.

4. Scheie AA. Modes of action currently known chemical antiplaque agents other than chlorhexdine. J Dent Res. 1989; 68(spec iss):1609–1616.

5. Kim SK, Song JH, Kim JB, Chang KW, Jeon JG. In vitro anticariogenic activity of Polygoni Radix. J Korean Acad Dent Health. 2005; 29:80–90.

6. Kang MS, Oh JS, Kang IC, Hong SJ, Choi CH. Inhibitory effect of methyl gallate and gallic acid on oral bacteria. J Microbiol. 2008; 46:744–750.

7. Chung DI, Ro JS, Chang KW. Antibacterial effect of some flavonoids against cariogenic bacteria. J Korean Acad Dent Health. 1996; 20:189–202.

8. Saeki Y, Kato T, Naito Y, Takazoe I, Okuda K. Inhibitory effects of funoran on the adherence and colonization of mutans streptococci. Caries Res. 1996; 30:119–125.

9. Kang MY, Jeong YH, Eun JB. Physical and chemical characteristics of flesh and pomace of japanese apricots (Prunus mume Sieb. Zucc). Korean J Food Sci Technol. 1999; 31:1434–1439.

10. Havsteen B. Flavonoids, a class of natural products of high pharmacological potency. Biochem Pharmacol. 1983; 32:1141–1148.

11. Lee HA, Nam ES, Park SI. Antimicrobial activity of maesil (Prunus mume) juice against selected pathogenic microorganisms. J Korean Soc Food Sci Nutr. 2003; 16:29–34.

12. Kim YS, Park YS, Lim MH. Antimicrobial activity of Prunus mume and schizandra chinenis H-20 extracts and their effects on qualityof functional kochujang. Korean J Food Sci Technol. 2003; 35:893–897.

13. You JS. Studies on antimicrobial activities of Kuwanon G isolated from the root bark of Morus alba L. against Streptococcus mutans [Doctoral dissertation]. Samcheok: Kangwon National University;2001. [Korean].

14. Katsura H, Tsukiyama RI, Suzuki A, Kobayashi M. In vitro antimicrobial activities of bakuchiol against oral microorganisms. Antimicrob Agents Chemother. 2001; 45:3009–3013.

15. Jo MJ, Min KJ. Anti-microbial activities against oral microbes and growth-inhibitory effect on oral tumor cell of extracts of perilla and mugwort. J Environ Health Sci. 2007; 33:115–122.

16. Choi BR, Kang JK, Kang KH. Antibacterial effects of extracts from citrus Peels. J Digit Converg. 2012; 10:559–564.

17. Yu HH, Seo SJ, Kim YH, Lee HY, Gum GC, Na JC, et al. Effects of methanol extract of aralia continentalis on the growth, acid production, adhesion, and insoluble glucan synthesis of Streptococcus mutans. Korean J Orient Physiol Pathol. 2005; 19:87–91.

18. Percival RS, Devine DA, Duggal MS, Chartron S, Marsh PD. The effect of cocoa polyphenols on the growth, metabolism, and biofilm formation by Streptococcus mutans and Streptococcus sanguinis. Eur J Oral Sci. 2006; 114:343–348.

19. Seo MH, Bae JH. Effect of butanol extracts from Prunus mume on the growth of salmonella typhimurium. Korean J Nutr. 2002; 35:926–931.

20. Jung JH. Inhibitory effect of Prunus mume extracts on the growth of gastric and colon cancer cell lines [master's thesis]. Daegu: Keimyung University;2000. [Korean].

21. Bae YK, Choe TB. Antioxidant and cell activity using extracts of mume fructus. Korean J Med Crop Sci. 2011; 19:388–394.

22. Ko BS, Park SK, Choi SB, Jun DW, Jang JS, Park SM. Hypoglycemic effects of crude extracts of Prunus mume. J Korean Soc Food Sci Nutr. 2004; 33:951–957.

23. Lee OH, Jung SH, Son JY. Antimicrobial activity of clove extract by extraction solvents. J Korean Soc Food Sci Nutr. 2004; 33:494–499.

24. Ha MH, Park WP, Lee SC, Heo HJ, Cho SH. Antimicrobial characteristic of methanolic extracts from Prunus mume byproducts against food spoilage microorganisms. Korean J Food Preserv. 2007; 14:183–187.

25. Lee ES, Han YS. Anti oralmicrobial activity of various extracts from parts of lotus (Nelumbo nucifera). Korean J Food Cookery Sci. 2011; 27:1–9.

26. Seneviratne CJ, Wong RW, Hägg U, Chen Y, Herath TD, Samaranayake PL, et al. Prunus mume extract exhibits antimicrobial activity against pathogenic oral bacteria. Int J Paediatr Dent. 2011; 21:299–305.

27. Zhanguo C, Jiuru L. Simultaneous and direct determination of oxalic acid, tartaric acid, malic acid, vitamin C, citric acid, and succinic acid in Fructus mume by reversed-phase high-performance liquid chromatography. J Chromatogr Sci. 2002; 40:35–39.

28. Hasegawa M. Flavonoids of various prunus species. J Org Chem. 1959; 24:408–409.

29. Kim GS. Studies on the antimicrobial activities and substances of Prunus mume [Doctoral dissertation]. Seoul: Ewha Womans University;1985. [Korean].

30. Chamberland S, Blais J, Hoang M, Dinh C, Cotter D, Bond E, et al. In vitro activities of RWJ-54428 (MC-02,479) against multi-resistant gram-positive bacteria. Antimicrob Agents Chemother. 2001; 45:1422–1430.