Exp Mol Med.  2017 Mar;49(3):e305. 10.1038/emm.2017.23.

From rabbit antibody repertoires to rabbit monoclonal antibodies

Affiliations
  • 1Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL, USA. crader@scripps.edu

Abstract

In this review, we explain why and how rabbit monoclonal antibodies have become outstanding reagents for laboratory research and increasingly for diagnostic and therapeutic applications. Starting with the unique ontogeny of rabbit B cells that affords highly distinctive antibody repertoires rich in in vivo pruned binders of high diversity, affinity and specificity, we describe the generation of rabbit monoclonal antibodies by hybridoma technology, phage display and alternative methods, along with an account of successful humanization strategies.


MeSH Terms

Antibodies, Monoclonal*
B-Lymphocytes
Bacteriophages
Humans
Hybridomas
Indicators and Reagents
Sensitivity and Specificity
Antibodies, Monoclonal
Indicators and Reagents
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