Lab Anim Res.  2017 Mar;33(1):8-14. 10.5625/lar.2017.33.1.8.

Comparison of commonly used ICR stocks and the characterization of Korl:ICR

Affiliations
  • 1Laboratory Animal Resources Division, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Cheongju, Korea. mwchung@korea.kr
  • 2Department of Life Sciences, Sogang University, Seoul 04107, Korea.

Abstract

Mouse is a commonly used animal in life science studies and is classified as outbred if genetically diverse and inbred if genetically homogeneous. Outbred mouse stocks, are used in toxicology, oncology, infection and pharmacology research. The National Institute of Food and Drug Safety Evaluation (NIFDS; former the Korea National Institute of Health) have bred ICR mice for more than 50 years. We investigated to provide users with information and promote accountability to the Korl:ICR. To secure the indigenous data, biological characteristics of Korl:ICR were identified by comparing with other ICR stocks. This domestic ICR stock was denominated as "˜Korl:ICR'. Phylogenetic analysis using SNPs indicated that the population stratification of the Korl:ICR was allocated different area with other ICR. In addition, we measured litter size, body weight, body length, various organ weight, hematology and clinical blood chemistry of the Korl:ICR compared to other ICR. Otherwise, there are no significant differences among the biological phenotypes of Korl:ICR and other ICR. These results suggest that as a genetically indigenous source colony, the Korl:ICR is seperated (or independent) stock with other ICR. Also, we confirmed that there is no difference among the Korl:ICR and other ICR on biological phenotypes. Therefore, the Korl:ICR source colony might be a new stock in distinction from other ICR, it is a good milestone in securing ownership of the national laboratory animal resource. The NIFDS expects that the Korl:ICR mice will be useful animal resource for our domestic researchers.

Keyword

Outbred; Korl:ICR stock; Rodent; Breeder

MeSH Terms

Animals
Animals, Laboratory
Biological Science Disciplines
Body Weight
Chemistry
Hematology
Korea
Litter Size
Mice
Mice, Inbred ICR
Organ Size
Ownership
Pharmacology
Phenotype
Polymorphism, Single Nucleotide
Population Characteristics
Rodentia
Social Responsibility
Toxicology

Figure

  • Figure 1 A phylogenetic tree showing the relationship between Korl:ICR and other ICR. The tree is unrooted. Values at nodes represent bootstrap support values (only values >50% are shown).

  • Figure 2 Physiological characteristics of Korl:ICR mice. (A) Body weights of ICR mice during 12 weeks. (B) Body length of ICR mice at the 12 weeks. (C-D) Percentage organ weight to body weight ratio of ICR mice (ANOVA, *P<0.05, **P<0.01, ***P<0.001, ns: not significant).


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Reference

1. Cui S, Chesson C, Hope R. Genetic variation within and between strains of outbred Swiss mice. Lab Anim. 1993; 27(2):116–123. PMID: 8501892.
Article
2. Rice MC, O'Brien SJ. Genetic variance of laboratory outbred Swiss mice. Nature. 1980; 283(5743):157–161. PMID: 7350540.
Article
3. Chia R, Achilli F, Festing MF, Fisher EM. The origins and uses of mouse outbred stocks. Nat Genet. 2005; 37(11):1181–1186. PMID: 16254564.
Article
4. Yamada J, Nikaido H, Matsumoto S. Genetic variability within and between outbred Wistar strains of rats. Jikken Dobutsu. 1979; 28(2):259–265. PMID: 477744.
Article
5. Phelan JP. Genetic variability and rodent models of human aging. Exp Gerontol. 1992; 27(2):147–159. PMID: 1521591.
Article
6. DeFries JC, Wilson JR, Erwin VG, Petersen DR. LS X SS recombinant inbred strains of mice: initial characterization. Alcohol Clin Exp Res. 1989; 13(2):196–200. PMID: 2658655.
Article
7. Feingold N, Feingold J, Mouton D, Bouthillier Y, Stiffel C, Biozzi G. Polygenic regulation of antibody synthesis to sheep erythrocytes in the mouse: a genetic analysis. Eur J Immunol. 1976; 6(1):43–51. PMID: 971719.
Article
8. Schlager G. Genetic Hypertension in the Mouse. Amsterdam: Elsevier;1994. p. 158–172.
9. Boutwell RK. Some Biological Aspects of Skin Carcinogenisis. Prog Exp Tumor Res. 1964; 4:207–250. PMID: 14150247.
10. Mathews CE, Bagley R, Leiter EH. ALS/Lt: a new type 2 diabetes mouse model associated with low free radical scavenging potential. Diabetes. 2004; 53(1 suppl):125–129.
Article
11. Garland T Jr, Morgan MT, Swallow JG, Rhodes JS, Girard I, Belter JG, Carter PA. Evolution of a small-muscle polymorphism in lines of house mice selected for high activity levels. Evolution. 2002; 56(6):1267–1275. PMID: 12144025.
Article
12. Kirkpatrick BW, Mengelt A, Schulman N, Martin IC. Identification of quantitative trait loci for prolificacy and growth in mice. Mamm Genome. 1998; 9(2):97–102. PMID: 9457667.
Article
13. Horvat S, Bünger L, Falconer VM, Mackay P, Law A, Bulfield G, Keightley PD. Mapping of obesity QTLs in a cross between mouse lines divergently selected on fat content. Mamm Genome. 2000; 11(1):2–7. PMID: 10602985.
Article
14. Crabbe JC, Belknap JK, Buck KJ. Genetic animal models of alcohol and drug abuse. Science. 1994; 264(5166):1715–1723. PMID: 8209252.
Article
15. Grahame NJ, Li TK, Lumeng L. Selective breeding for high and low alcohol preference in mice. Behav Genet. 1999; 29(1):47–57. PMID: 10371758.
16. Lynch CJ. The so-called Swiss mouse. Lab Anim Care. 1969; 19(2):214–220. PMID: 4240230.
17. Keenan KP, Smith PF, Hertzog P, Soper K, Ballam GC, Clark RL. The effects of overfeeding and dietary restriction on Sprague-Dawley rat survival and early pathology biomarkers of aging. Toxicol Pathol. 1994; 22(3):300–315. PMID: 7817120.
Article
18. Keenan KP, Ballam GC, Dixit R, Soper KA, Laroque P, Mattson BA, Adams SP, Coleman JB. The effects of diet, overfeeding and moderate dietary restriction on Sprague-Dawley rat survival, disease and toxicology. J Nutr. 1997; 127(5 suppl):851S–856S. PMID: 9164252.
Article
19. Kacew S, Ruben Z, McConnell RF. Strain as a determinant factor in the differential responsiveness of rats to chemicals. Toxicol Pathol. 1995; 23(6):701–714. PMID: 8772256.
20. Kacew S, Ruben Z. Importance of confounding factors including strain, sex and diet in modeling chemically-induced toxicity. Toxicol Ecotoxicol News. 1997; 4:158–160.
21. Snell GD. Biology of the Laboratory Mouse. 1st Edn. McGraw-Hill: New York, MA, US;1941.
22. Brown SD, Moore MW. The International Mouse Phenotyping Consortium: past and future perspectives on mouse phenotyping. Mamm Genome. 2012; 23(9-10):632–640. PMID: 22940749.
Article
23. O'Connor JC, Lawson MA, André C, Moreau M, Lestage J, Castanon N, Kelley KW, Dantzer R. Lipopolysaccharide-induced depressive-like behavior is mediated by indoleamine 2,3-dioxygenase activation in mice. Mol Psychiatry. 2009; 14(5):511–522. PMID: 18195714.
24. Zhong SZ, Ge QH, Qu R, Li Q, Ma SP. Paeonol attenuates neurotoxicity and ameliorates cognitive impairment induced by d-galactose in ICR mice. J Neurol Sci. 2009; 277(1-2):58–64. PMID: 19007942.
Article
25. Song SH, Kim JE, Go J, Koh EK, Sung JE, Lee HA, Choi KM, Kim HD, Jung YS, Kim KS, Hwang DY. Comparison of the response using ICR mice derived from three different sources to ethanol/hydrochloric acid-induced gastric injury. Lab Anim Res. 2016; 32(1):56–64. PMID: 27051443.
Article
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