Biomol Ther.  2016 Nov;24(6):659-664. 10.4062/biomolther.2016.021.

Antiplatelet and Antithrombotic Effects of the Extract of Lindera obtusiloba Leaves

• Kim JH
• Lee J
• Kang S
• Moon H
• Chung KH
• Kim KR

• KMID: 2374621
• DOI: http://doi.org/10.4062/biomolther.2016.021

Abstract

Lindera obtusiloba has been used in traditional herbal medicine for the treatment of blood stasis and inflammation. The leaves of Lindera obtusiloba have been reported to exhibit various physiological activities. However, there is little information available on their antiplatelet and antithrombotic activities. Thus, the present study aimed to evaluate the effect of Lindera obtusiloba leaf extract (LLE) on platelet activities, coagulation and thromboembolism. In a platelet aggregation study, LLE significantly inhibited various agonist-induced platelet aggregations in vitro and ex vivo. Furthermore, LLE significantly inhibited collagen-induced thromboxane A2 (TXA2) production in rat platelets. In addition, oral administration of LLE was protective in a mouse model of pulmonary thromboembolism induced by intravenous injection of a mixture of collagen and epinephrine. Interestingly, LLE did not significantly alter prothrombin time (PT) and activated partial thromboplastin time (aPTT). This study indicates that the antithrombotic effects of LLE might be due to its antiplatelet activities rather than anticoagulation. Taken together, these results suggest that LLE may be a candidate preventive and therapeutic agent in cardiovascular diseases associated with platelet hyperactivity.

Keyword

Lindera obtusiloba; Platelet aggregation; Thrombosis; Thromboxane

MeSH Terms

Administration, Oral
Animals
Blood Platelets
Cardiovascular Diseases
Collagen
Epinephrine
Herbal Medicine
In Vitro Techniques
Inflammation
Injections, Intravenous
Lindera*
Mice
Partial Thromboplastin Time
Platelet Aggregation
Prothrombin Time
Pulmonary Embolism
Rats
Thromboembolism
Thrombosis
Thromboxane A2
Collagen
Epinephrine
Thromboxane A2