Korean J Ophthalmol.  2016 Jun;30(3):172-179. 10.3341/kjo.2016.30.3.172.

Oxidative Stress Levels in Aqueous Humor from High Myopic Patients

• Kim EB
• Kim HK
• Hyon JY
• Wee WR
• Shin YJ

• KMID: 2373972
• DOI: http://doi.org/10.3341/kjo.2016.30.3.172

Abstract

PURPOSE
To compare oxidative stress status in the aqueous humor of highly myopic eyes and control eyes.
METHODS
Aqueous humor samples were collected from 15 highly myopic eyes (high myopia group) and 23 cataractous eyes (control group) during cataract surgery. Central corneal thickness, corneal endothelial cell density, hexagonality of corneal endothelial cells, and cell area of corneal endothelial cells were measured using specular microscopy. Axial length was measured using ultrasound biometry. 8-Hydroxydeoxyguanosine (8-OHdG) and malondialdehyde levels were measured using enzyme-linked immunosorbent assay.
RESULTS
8-OHdG level was lower in the aqueous humor of myopic patients than in that of control group (p = 0.014) and was positively correlated with central corneal thickness and negatively correlated with axial length (r = 0.511, p = 0.02; r = -0.382, p < 0.001). There was no correlation between 8-OHdG level and corneal endothelial cell density, hexagonality, or cell area. Malondialdehyde level did not show any correlation with any parameters evaluated.
CONCLUSIONS
8-OHdG might be a sensitive biomarker for evaluating oxidative stress status in the eye. Oxidative stress level was lower in the aqueous humor of highly myopic eyes compared to that in control eyes, which indicates lower metabolic activity in these eyes.

Keyword

8-Hydroxydeoxyguanosine; Axial eye length; Corneal pachymetry; High myopia; Malondialdehyde

MeSH Terms

Aged
Aqueous Humor/*metabolism
Deoxyguanosine/*analogs & derivatives/metabolism
Enzyme-Linked Immunosorbent Assay
Female
Humans
Male
Malondialdehyde/*metabolism
Middle Aged
Myopia/*metabolism/physiopathology
*Oxidative Stress
Refraction, Ocular/*physiology
Severity of Illness Index
Deoxyguanosine
Malondialdehyde

Figure

• 

  Fig. 1 Oxidative stress marker levels in aqueous humor and axial length (AXL). (A) 8-Hydroxydeoxyguanosine (8-OHdG) level in aqueous humor was lower in the high myopia group compared to the control group (p = 0.014, Mann-Whitney U-test). (B) Malondialdehyde (MDA) levels were not different between control and high myopia groups. (C) 8-OHdG level decreased depending on AXL (r = -0.382, p < 0.001, Spearman's correlation coefficient). (D) MDA level did not correlate with AXL. *Statistically significant.

• 

  Fig. 2 Correlation between 8-hydroxydeoxyguanosine (8-OHdG) and corneal endothelial cells. (A) 8-OHdG level was positively correlated with central corneal thickness (CCT) (r = 0.511, p = 0.02, Spearman's correlation coefficient). (B) 8-OHdG level did not correlate with corneal endothelial cell density (CECD). (C) 8-OHdG level did not correlate with cell hexagonality. (D) 8-OHdG level did not correlate with cell area. *Statistically significant by Spearman's correlation coefficient test.

• 

  Fig. 3 Correlation between malondialdehyde (MDA) and corneal endothelial cells. (A) MDA level did not correlate with central corneal thickness (CCT). (B) MDA level did not correlate with corneal endothelial cell density (CECD). (C) MDA level did not correlate with cell hexagonality. (D) MDA level did not correlate with cell area.

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