Mycobiology.  2016 Dec;44(4):291-301. 10.5941/MYCO.2016.44.4.291.

Evaluation of Antioxidant, Anti-cholinesterase, and Anti-inflammatory Effects of Culinary Mushroom Pleurotus pulmonarius

• Nguyen TK
• Im KH
• Choi J
• Shin PG
• Lee TS

• KMID: 2366454
• DOI: http://doi.org/10.5941/MYCO.2016.44.4.291

Abstract

Culinary mushroom Pleurotus pulmonarius has been popular in Asian countries. In this study, the anti-oxidant, cholinesterase, and inflammation inhibitory activities of methanol extract (ME) of fruiting bodies of P. pulmonarius were evaluted. The 1,1-diphenyl-2-picryl-hydrazy free radical scavenging activity of ME at 2.0 mg/mL was comparable to that of butylated hydroxytoluene, the standard reference. The ME exhibited significantly higher hydroxyl radical scavenging activity than butylated hydroxytoluene. ME showed slightly lower but moderate inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase than galantamine, a standard AChE inhibitor. It also exhibited protective effect against cytotoxicity to PC-12 cells induced by glutamate (10~100 µg/mL), inhibitory effect on nitric oxide (NO) production and inducible nitric oxide synthase protein expression in lipopolysaccharide-stimulated RAW 264.7 macrophages, and carrageenan-induced paw edema in a rat model. High-performance liquid chromatography analysis revealed the ME of P. pulmonarius contained at least 10 phenolic compounds and some of them were identified by the comparison with known standard phenolics. Taken together, our results demonstrate that fruiting bodies of P. pulmonarius possess antioxidant, anti-cholinesterase, and inflammation inhibitory activities.

Keyword

Anti-cholinesterase; Anti-inflammation; Antioxidant; Cytotoxicity; Pleurotus pulmonarius

MeSH Terms

Acetylcholinesterase
Agaricales*
Asian Continental Ancestry Group
Butylated Hydroxytoluene
Butyrylcholinesterase
Cholinesterases
Chromatography, Liquid
Edema
Fruit
Galantamine
Glutamic Acid
Humans
Hydroxyl Radical
Inflammation
Macrophages
Methanol
Models, Animal
Nitric Oxide
Nitric Oxide Synthase Type II
Phenol
Pleurotus*
Acetylcholinesterase
Butylated Hydroxytoluene
Butyrylcholinesterase
Cholinesterases
Galantamine
Glutamic Acid
Hydroxyl Radical
Methanol
Nitric Oxide
Nitric Oxide Synthase Type II
Phenol