Korean J Parasitol.  2015 Dec;53(6):777-783. 10.3347/kjp.2015.53.6.777.

Pathological Lesions and Inducible Nitric Oxide Synthase Expressions in the Liver of Mice Experimentally Infected with Clonorchis sinensis

Affiliations
  • 1National Institute of Parasitic Disease, Chinese Center for Disease Control and Prevention; Key Laboratory of Parasite and Vector Biology, Ministry of Health; WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, P. R. Chi
  • 2Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control; Guangxi Key Laboratory for the Prevention and Control of Viral Hepatitis, Nanning 530028, P. R. China.
  • 3Department of Parasitology, Guangxi Medical University, Nanning 530021, P. R. China.

Abstract

The nitric oxide (NO) formation and intrinsic nitrosation may be involved in the possible mechanisms of liver fluke-associated carcinogenesis. We still do not know much about the responses of inducible NO synthase (iNOS) induced by Clonorchis sinensis infection. This study was conducted to explore the pathological lesions and iNOS expressions in the liver of mice with different infection intensity levels of C. sinensis. Extensive periductal inflammatory cell infiltration, bile duct hyperplasia, and fibrosis were commonly observed during the infection. The different pathological responses in liver tissues strongly correlated with the infection intensity of C. sinensis. Massive acute spotty necrosis occurred in the liver parenchyma after a severe infection. The iNOS activity in liver tissues increased, and iNOS-expressing cells with morphological differences were observed after a moderate or severe infection. The iNOS-expressing cells in liver tissues had multiple origins.

Keyword

Clonorchis sinensis; pathology; inducible nitric oxide synthase; liver; immunohistochemistry

MeSH Terms

Animals
Clonorchiasis/*enzymology/genetics/parasitology/*pathology
Clonorchis sinensis/*physiology
Female
Humans
Liver/*enzymology/parasitology/pathology
Mice
Mice, Inbred BALB C
Nitric Oxide Synthase Type II/*genetics/metabolism
Nitric Oxide Synthase Type II
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