Ann Dermatol.  1998 Jul;10(3):147-152. 10.5021/ad.1998.10.3.147.

Ultraviolet B-Induced Apoptosis of Normal Human Melanocytes and G361 Cells

Abstract

BACKGROUND
Ultraviolet(UV) light is one of the injurious environmental agents which is known to lead to apoptosis of cells. However, studies on UVB-induced apoptosis of melanocytes are still lacking and there are some discrepancies between researchers.
OBJECTIVE
Our purpose was to evaluate the characteristics of UVB-induced apoptosis of melanocytes and G361 cells.
METHODS
Cultured normal human melanocytes and malignant melanoma cell lines (G361 cells) were analyzed by several detection methods including morphological examination of propidium iodide(PI) stained cells under fluorescence microscopy, quantitation of fragmented DNA, and flow cytometric analysis.
RESULTS
Both melanocytes and G361 cells showed similar rate of apoptosis with gradual increment of UVB doses by the quantitation of fragmented DNA. However, flow cytometric analysis using scatter properties and PI stainability revealed that the melanocytes were more resistant to UVB than G361 cells.
CONCLUSION
We suggest that melanocytes seem to be more resistant to UVB-induced injury than G361 cells. In addition, various methods for the detection of apoptosis might be necessary for its study. (Ann Dermatol 10:(3) 147152, 1998).

Keyword

Melanocyte; G361 cell; Ultraviolet B; Apoptosis

MeSH Terms

Apoptosis*
Cell Line
DNA
Humans*
Melanocytes*
Melanoma
Microscopy, Fluorescence
Propidium
DNA
Propidium
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