Nat Prod Sci.  2016 Sep;22(3):154-161. 10.20307/nps.2016.22.3.154.

Chemical Constituents from Leaves of Pileostegia viburnoides Hook.f.et Thoms

• Li XJ
• Liu ZZ
• Kim KW
• Wang X
• Li Z
• Kim YC
• Yook CS
• Liu XQ

• KMID: 2355139
• DOI: http://doi.org/10.20307/nps.2016.22.3.154

Abstract

Phytochemical investigation on the leaves of Pileostegia viburnoides Hook.f.et Thoms led to the isolation of twenty-five compounds, and their structures were identified as n-dotriacontane (1), taraxeryl acetate (2), friedelin (3), epifriedelinol (4), canophyllal (5), stigmast-4-en-3-one (6), stigmasterol (7), (24R)-5A-stigmastane-3,6-dione (8), ursolic acid (9), pomolic acid (10), umbelliferone (11), 4-epifriedelin (12), n-octatriacontanol (13), β-amyrin (14), α-amyrin (15), taraxerol (16), nonadecanol (17), friedelane (18), arachic acid (19), protocatechuic acid (20), n-pentatriacontanol (21), hexadecanoic acid (22), vincosamide (23), daucosterol (24), and skimming (25), respectively. To our best knowledge, compounds 1, 2, 12, 13, 17 - 19 and 21-23 were new within Saxifragaceae family. Compounds 15, 16, and 20 were produced from this genus for the first time. Compounds 4, 14 and 25 were first obtained from species P. viburnoides and compounds 3, 5 - 11, and 24 were achieved from the leaves of P. viburnoides for the first time. Furthermore, the anti-neuroinflammatory activity of these isolates was evaluated.

Keyword

Pileostegia viburnoides Hook.f.et Thoms; Triterpenes; 4-epifriedelin; Coumarins; Vincosamide; Chemical constituents; Anti-neuroinflammatory

MeSH Terms

Coumarins
Humans
Palmitic Acid
Saxifragaceae
Stigmasterol
Triterpenes
Coumarins
Palmitic Acid
Stigmasterol
Triterpenes

Figure

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  Fig. 1. Chemical structures of the isolated compounds 1–25.

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  Fig. 2. Effects of selected compounds 2-5, 11, 12, 16, 18, 23, and 25 on productions of NO in LPS-stimulated BV2 microglias. Cells were pretreated for 30 min with the indicated concentrations of selected compounds, then stimulated for 24 h with LPS (1 µg/mL). The concentrations of nitrite were determined as described in the Experimental Section. Data represent the mean values of three experiments ± SD.

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