Clin Exp Otorhinolaryngol.  2016 Sep;9(3):270-277. 10.21053/ceo.2015.01305.

The Expression and Relationship of CD68-Tumor-Associated Macrophages and Microvascular Density With the Prognosis of Patients With Laryngeal Squamous Cell Carcinoma

Affiliations
  • 1Department of Otorhinolaryngology, Qilu Hospital of Shandong University, Jinan, China. XinLiangPancn@126.com
  • 2Department of Otorhinolaryngology, The Affiliated Hospital of Weifang Medical University, Weifang, China.
  • 3Department of Pathology, The Affiliated Hospital of Weifang Medical University, Weifang, China.

Abstract


OBJECTIVES
We sought to identify the expression of CD68-tumor-associated macrophages (TAMs) and CD34-microvascular density (MVD) in laryngeal squamous cell carcinoma (LSCC), to study the relationship with clinical pathological parameters and to determine whether their expression is predictive of disease.
METHODS
Pathologically confirmed 45 LSCC tissue and 20 peritumoral non-tumor tissue were examined. Immunohistochemical studies were used to detect the expression of CD68-TAMs and CD34-MVD.
RESULTS
The positive expression rate of CD68 in LSCC tissue was 82% (37/45), which was higher than the 10% (2/20) expression rate of the peritumoral tissue (P<0.05). The CD34-MVD positive expression rate in the LSCC tissue was 26.5±6.4, which obviously higher than 12.2±4.0 expression rate of the peritumoral tissue (P<0.05). The positive expression rates of both CD68 and CD34-MVD were higher in the lymph node metastasis (LNM) positive group than in the LNM negative group. The expression of CD68 had positive correlation with CD34-MVD. The 5-year disease-free survival rate in the group with the low CD68 expression was significantly higher than that in the group with high CD68 expression (76% vs. 42%, respectively).
CONCLUSION
The high expression of CD68-TAMs in LSCC and its positive correlation with CD34-MVD illustrates that both play an important role in promoting the metastasis and angiogenesis of this cancer. Their expression was also positively correlated with the prognoses of these patients, suggesting that they could be used as important prognostic markers for LSCC.

Keyword

Laryngeal Neoplasms; Tumor-Associated Macrophages; CD; Microvascular Density

MeSH Terms

Carcinoma, Squamous Cell*
Disease-Free Survival
Epithelial Cells*
Humans
Laryngeal Neoplasms
Lymph Nodes
Macrophages*
Neoplasm Metastasis
Prognosis*

Figure

  • Fig. 1. (A) Expression of CD68 in laryngeal squamous cell carcinoma SP (×200). (B) Expression of CD68 in laryngeal squamous cell carcinoma SP (×400). (C) Little or no expression in adjacent non-caner normal tissues SP (×200). (D) Expression of CD34 in tumor nests edge zone SP (×200). (E) Expression of CD34 in Laryngeal squamous cell carcinoma of the inside edge of tumor tissue stroma SP (×200). SP, streptavidin-perosidase.

  • Fig. 2. The survival curves in the low infiltration area (low expression zone) and high infiltration area (highly expression area) of CD68 in laryngeal squamous carcinoma. The average survival time was 106.1±10.2 months in CD68 low-expression area, 5-year survival rate was 76% (16/21). The average survival time was 56.3±9.1 months in the high-expression area, 5-year survival rate was 42% (10/24). There was statistical significance between two by log-rank method (P=0.020).


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