Clin Exp Otorhinolaryngol.  2016 Sep;9(3):270-277. 10.21053/ceo.2015.01305.

The Expression and Relationship of CD68-Tumor-Associated Macrophages and Microvascular Density With the Prognosis of Patients With Laryngeal Squamous Cell Carcinoma

  • 1Department of Otorhinolaryngology, Qilu Hospital of Shandong University, Jinan, China.
  • 2Department of Otorhinolaryngology, The Affiliated Hospital of Weifang Medical University, Weifang, China.
  • 3Department of Pathology, The Affiliated Hospital of Weifang Medical University, Weifang, China.


We sought to identify the expression of CD68-tumor-associated macrophages (TAMs) and CD34-microvascular density (MVD) in laryngeal squamous cell carcinoma (LSCC), to study the relationship with clinical pathological parameters and to determine whether their expression is predictive of disease.
Pathologically confirmed 45 LSCC tissue and 20 peritumoral non-tumor tissue were examined. Immunohistochemical studies were used to detect the expression of CD68-TAMs and CD34-MVD.
The positive expression rate of CD68 in LSCC tissue was 82% (37/45), which was higher than the 10% (2/20) expression rate of the peritumoral tissue (P<0.05). The CD34-MVD positive expression rate in the LSCC tissue was 26.5±6.4, which obviously higher than 12.2±4.0 expression rate of the peritumoral tissue (P<0.05). The positive expression rates of both CD68 and CD34-MVD were higher in the lymph node metastasis (LNM) positive group than in the LNM negative group. The expression of CD68 had positive correlation with CD34-MVD. The 5-year disease-free survival rate in the group with the low CD68 expression was significantly higher than that in the group with high CD68 expression (76% vs. 42%, respectively).
The high expression of CD68-TAMs in LSCC and its positive correlation with CD34-MVD illustrates that both play an important role in promoting the metastasis and angiogenesis of this cancer. Their expression was also positively correlated with the prognoses of these patients, suggesting that they could be used as important prognostic markers for LSCC.


Laryngeal Neoplasms; Tumor-Associated Macrophages; CD; Microvascular Density

MeSH Terms

Carcinoma, Squamous Cell*
Disease-Free Survival
Epithelial Cells*
Laryngeal Neoplasms
Lymph Nodes
Neoplasm Metastasis
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