Anat Cell Biol.  2016 Sep;49(3):177-183. 10.5115/acb.2016.49.3.177.

Macrophage density in pharyngeal and laryngeal muscles greatly exceeds that in other striated muscles: an immunohistochemical study using elderly human cadavers

• Rhee S
• Yamamoto M
• Kitamura K
• Masaaki K
• Katori Y
• Murakami G
• Abe SI

• KMID: 2353342
• DOI: http://doi.org/10.5115/acb.2016.49.3.177

Abstract

Macrophages play an important role in aging-related muscle atrophy (i.e., sarcopenia). We examined macrophage density in six striated muscles (cricopharyngeus muscle, posterior cricoarytenoideus muscle, genioglossus muscle, masseter muscle, infraspinatus muscle, and external anal sphincter). We examined 14 donated male cadavers and utilized CD68 immunohistochemistry to clarify macrophage density in muscles. The numbers of macrophages per striated muscle fiber in the larynx and pharynx (0.34 and 0.31) were 5-6 times greater than those in the tongue, shoulder, and anus (0.05-0.07) with high statistical significance. Thick muscle fibers over 80 µm in diameter were seen in the pharynx, larynx, and anal sphincter of two limited specimens. Conversely, in the other sites or specimens, muscle fibers were thinner than 50 µm. We did not find any multinuclear muscle cells suggestive of regeneration. At the beginning of the study, we suspected that mucosal macrophages might have invaded into the muscle layer of the larynx and pharynx, but we found no evidence of inflammation in the mucosa. Likewise, the internal anal sphincter (a smooth muscle layer near the mucosa) usually contained fewer macrophages than the external sphincter. The present result suggest that, in elderly men, thinning and death of striated muscle fibers occur more frequently in the larynx and pharynx than in other parts of the body.

Keyword

Deglutition; Deglutition disorders; Sarcopenia; Larynx; Pharynx

MeSH Terms

Aged*
Anal Canal
Cadaver*
Deglutition
Deglutition Disorders
Humans
Humans*
Immunohistochemistry
Inflammation
Laryngeal Muscles*
Larynx
Macrophages*
Male
Masseter Muscle
Mucous Membrane
Muscle Cells
Muscle, Smooth
Muscle, Striated*
Muscles
Muscular Atrophy
Pharynx
Regeneration
Sarcopenia
Shoulder
Tongue

Figure

• 

  Fig. 1 Histological section for assessment of the pharyngeal and laryngeal striated muscles. A 72-year-old man. Cross-sections (elastica Masson staining) of the striated muscles are easily found in tilted horizontal sections including the cricothyroid joint (CT joint). CC, cricoid cartilage; CPM, cricopharyngeal muscle; ICTC, inferior cornu of the thyroid cartilage; PCAM, posterior cricoarytenoid muscle; TYG, thyroid gland. Scale bar=5 mm.

• 

  Fig. 2 Macrophages in cross-sections of striated muscles. All panels are prepared at the same magnification (scale bar in panel B=0.1 mm). Each panel corresponds to almost half the area of the visual field employing a ×20 objective for counting of macrophages. Arrows and arrowheads indicate CD68-positive macrophages: some macrophages (arrowheads) are large and appear to be active. Pharynx, the cricopharyngeal muscle (A); larynx, the posterior cricoarytenoid muscle (B); tongue, the genioglossus muscle (C); face, masseter muscle (D); shoulder, the infraspinatus muscle (E); anus, the external anal sphincter muscle (F).

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