Ewha Med J.  2003 Jun;26(2):143-148. 10.12771/emj.2003.26.2.143.

Neurotoxic Effect of beta-Amyloid Peptide in Hippocampal Slice Culture

Affiliations
  • 1Department of Pharmacology and Medical Research Mstitute, College of Medicine, Ewha Women University, Korea.

Abstract

Alzheimer's disease(AD) is primarily characterized by neurofibrillary tangles, senile plaques, and neurodegeneration. The major component of senile plaques is the beta-amyloid peptide(A beta), Which is considered to have a causal role in AD. However, the biological activities of Abeta in AD has not been clearly defined. In this study we have investigated the effects of Abeta 25-35 fragment to neurons using organotypic hippocampal slice culture system which maintained intact hippocampal synaptic circuit and anatomy. Hippocampal slice culture is prepared from rat postnatal 10-old days and after 14 days culture, slices were treated with 10uM Abeta 25-35 fragment. Neuronal death was measured with propidium iodide(PI) uptake and NeuN, neuronal marker, staining. After treatment of Abeta 25-35 fragment for 3days or 7days on hippocampal slice culture, we observed the increased PI uptake and the decreased number of NeuN-stained neuron in CA1 region of hippocampal pyramidal layer or dentate gyrus. These results suggested that Abeta 25-35 fragment exerts the neurotoxicity in hippocampal slice culture.

Keyword

Beta-Amyloid peptide; Neurotoxicity; Hippocampal slice culture
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