J Vet Sci.  2015 Sep;16(3):325-331. 10.4142/jvs.2015.16.3.325.

The potential molecular effects of bursal septpeptide II on immune induction and antitumor activity

• Zhou GF
• Liu QT
• Zhou B
• Qiu YF
• Liu XD
• Ma ZY
• Feng XL
• Cao RB
• Chen PY

• KMID: 2344305
• DOI: http://doi.org/10.4142/jvs.2015.16.3.325

Abstract

The bursa of Fabricius (BF) is the acknowledged central humoral immune organ in birds. Bursal septpeptide II (BSP-II) is an immunomodulatory bioactive peptide isolated from BF. To understand the effects of BSP-II on immune induction, gene expression profiles of hybridoma cells treated with BSP-II were evaluated. Pathway analysis showed that regulated genes were involved in cytokine-cytokine receptor interactions, T cell receptor signaling pathway, and pathway in cancer. It was observed that BSP-II reduced tumor cells proliferation and stimulated p53 expression. These results indicate potential mechanisms underlying the effects of the humoral immune system on immune induction, including antitumor activities. Our study has provided a novel insight into immunotherapeutic strategies for treating human tumors.

Keyword

bursal septpeptide II; humoral immune system; pathway analysis; tumor suppressor p53

MeSH Terms

Animals
Antineoplastic Agents/*pharmacology
Avian Proteins/*pharmacology
Bursa of Fabricius/immunology
Cell Proliferation/drug effects
Chickens/*immunology
Hybridomas/drug effects
Immunologic Factors/*pharmacology
Oligonucleotide Array Sequence Analysis/veterinary
Signal Transduction/*drug effects
*Transcriptome
Antineoplastic Agents
Avian Proteins
Immunologic Factors

Figure

• 

  Fig. 1 The impact of bursal septpeptide II (BSP-II) on hybridoma cells. (A) After incubation with the indicated concentrations of BSP-II for 48 h, concentrations of antibody in secreted into the cell supernatant were measured with ELISA method. (B) Cell viability was also measured with MTT assay. Bars represent the mean ± standard deviation (SD) of three independent experiments. *p < 0.05 and **p < 0.01 compared to the control.

• 

  Fig. 2 The antiproliferative effect of BSP-II on tumor cells. After 48 h of treatment with BSP-II, viability of tumor (A) and normal (B) cells was evaluated with MTT assay, respectively. Bars represent the mean ± SD of three independent experiments. *p < 0.05 and **p < 0.01 compared to the control.

• 

  Fig. 3 p53 transcription and protein expression after BSP-II treatment. Vero cells were transfected with p53 Luc and pRL-TK plasmid, and then cultured with or without BSP-II for 24 h. p53-luciferase activity levels were then measured (A). The transfected Vero cells were also pre-incubated with α-pifithrin for 2 h, incubated with or without BSP-II (2 µg/mL) for 22 h, and the level of p53-luciferase activity was measured (B). Non-transfected Vero cells were cultured with or without BSP-II for 24 h, and Western blotting analysis was performed to detect p53 and Bax protein expressions (C). Transfected or non-transfected Vero cells were treated with Dox as a positive control. Bars represent the mean ± SD of three independent experiments. *p < 0.05 and **p < 0.01 compared to the control. Dox: doxorubicin.

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