J Korean Ophthalmol Soc.  2010 Mar;51(3):313-319.

CCL4 Concentration in Tears of Dry Eye Patients and Its Correlation With Tear Surface Parameters

Affiliations
  • 1Department of Ophthalmology, Chonnam National University Medical School, Gwangju, Korea. kcyoon@chonnam.ac.kr

Abstract

PURPOSE
To investigate the expression of CCL4/MIP-1beta in tears of patients with dry eye syndrome and its correlation with the tear surface parameters.
METHODS
Forty patients with dry eye syndrome (seven Meibomian gland dysfunction and 33 non-Meibomian gland dysfunction patients) and 25 control subjects were recruited. The concentrations of CCL4 in tears were measured using an enzyme-linked immunosorbent assay. The correlations between CCL4 levels and tear surface parameters including tear film break-up time (BUT), basal tear secretion (BST), tear clearance test (TCR), corneal sensation, keratoepitheliopathy (KEP) and conjunctival goblet cell density (GCD) were analyzed in patients with dry eye syndrome.
RESULTS
The mean levels of MIP-1beta were 4.3+/-5.2 pg/ml in patients with dry eye syndrome and 81.6+/-294.3 pg/ml in control subjects (p=0.03). The mean levels in non-Meibomian gland disease patients (90.4+/-313.9 pg/ml) were significantly higher than those of Meibomian gland dysfunction patients (20.3+/-23.2 pg/ml, p=0.02). CCL4 levels correlated significantly with BST, TCR, KEP and GCD (p<0.05).
CONCLUSIONS
The level of MIP-1beta is elevated in tears of patients with dry eye syndrome and correlates with disease severity and tear surface parameters.

Keyword

CCL4; Chemokine; Dry eye syndrome; Tear surface parameter

MeSH Terms

Chemokine CCL4
Dry Eye Syndromes
Enzyme-Linked Immunosorbent Assay
Eye
Goblet Cells
Humans
Meibomian Glands
Sensation
Tears
Chemokine CCL4

Figure

  • Figure 1. CCL4 levels in tears of Meibomian gland dysfunction patients, non-Mebomian gland dysfunction patients and control subjects. DES=dry eye syndrome; MGD=Meibomian gland dysfunction; * p<0.05.

  • Figure 2. Correlation between MIP-1β levels in tears of dry eye patients and tear surface parameters including tear break-up time (A), basal tear secretion (B), tear clearance rate (C), corneal sensation (D), keratoepitheliopathy score (E), and conjunctival goblet cell density(F).


Reference

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